Near infrared chemical imaging microscope
First Claim
1. A near infrared radiation chemical imaging system comprising:
- a) an illumination source for illuminating an area of a sample using light in the near infrared radiation wavelength;
b) a device for collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, emitted or scattered from said illuminated area of said sample and producing a collimated beam therefrom;
c) a near infrared imaging spectrometer for selecting a near infrared radiation image of said collimated beam; and
d) a detector for collecting said filtered near infrared images.
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Abstract
A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, or emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation images of the collimated beam. The spectrometer comprises a liquid crystal tunable filter. The filtered selected images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.
53 Citations
16 Claims
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1. A near infrared radiation chemical imaging system comprising:
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a) an illumination source for illuminating an area of a sample using light in the near infrared radiation wavelength;
b) a device for collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, emitted or scattered from said illuminated area of said sample and producing a collimated beam therefrom;
c) a near infrared imaging spectrometer for selecting a near infrared radiation image of said collimated beam; and
d) a detector for collecting said filtered near infrared images. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A chemical imaging system comprising:
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a) an illumination source for illuminating an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength;
b) a device for collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, or emitted or scattered from said illuminated area of said sample and producing a collimated beam therefrom;
c) a near infrared imaging spectrometer for selecting a near infrared radiation images of said collimated beam, wherein the spectrometer comprises a liquid crystal tunable filer;
d) detector for collecting said filtered selected near infrared images; and
e) a device for detecting said visible wavelength light from said illuminated area of said sample.
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13. A chemical imaging method comprising the steps of:
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a) illuminating an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength;
b) collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, or emitted or scattered from said illuminated area of said sample and producing a collimated beam therefore;
c) filtering said collimated beam to produce a near infrared radiation images of said collimated beam while simultaneously detecting said optical visible wavelength light from said illuminated area of said sample, wherein the filtering is performed using a liquid crystal tunable filter;
d) collecting said filtered near infrared images; and
e) processing said collected near infrared images to produce and display a chemical image of said sample.
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14. A method for producing a volumetric image of a sample comprising the steps of:
- a) incorporating a refractive image formation optic exhibiting a chromatic response in the optical path of the microscope before the near infrared detector;
b) collecting images of said sample at a plurality of near infrared wavelengths through said objective at a fixed focus condition; and
c) processing said collected images to reconstruct and display a depth resolved image of said sample.
- a) incorporating a refractive image formation optic exhibiting a chromatic response in the optical path of the microscope before the near infrared detector;
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15. A method for chemically analyzing a sample comprising the steps of:
- a) seeding said sample with a plurality of analytes having at least one of a known composition, structure and concentration;
b) collecting a plurality of spatially-resolved spectra for said plurality of analytes;
c) producing a plurality of chemical images of said sample containing said plurality of analytes; and
d) processing said plurality of chemical images to generate a chemical image of said sample, and displaying said chemical image. - View Dependent Claims (16)
- a) seeding said sample with a plurality of analytes having at least one of a known composition, structure and concentration;
Specification