Compositions for regeneration and repair of cartilage lesions
First Claim
Patent Images
1. A product for repair of cartilage lesions, comprising:
- a. a cartilage repair matrix suitable for conforming to a defect in cartilage; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
transforming growth factor β
1 (TGFβ
1), bone morphogenetic protein (BMP)-2, BMP-3, and BMP-7;
wherein the quantity of said TGFβ
1 in said mixture is greater than 1% of total proteins in said mixture;
wherein the quantity of said BMP-2 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
wherein the quantity of said BMP-3 in said mixture is from about 0.1% to about 15% of total proteins in said mixture; and
, wherein the quantity of said BMP-7 in said mixture is from about 0.01% to about 10% of total proteins in said mixture.
0 Assignments
0 Petitions
Accused Products
Abstract
Disclosed is a cartilage repair product that induces both cell ingrowth into a bioresorbable material and cell differentiation into cartilage tissue. Such a product is useful for regenerating and/or repairing both vascular and avascular cartilage lesions, particularly articular cartilage lesions, and even more particularly mensical tissue lesions, including tears as well as segmental defects. Also disclosed is a method of regenerating and repairing cartilage lesions using such a product.
186 Citations
80 Claims
-
1. A product for repair of cartilage lesions, comprising:
-
a. a cartilage repair matrix suitable for conforming to a defect in cartilage; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
transforming growth factor β
1 (TGFβ
1), bone morphogenetic protein (BMP)-2, BMP-3, and BMP-7;
wherein the quantity of said TGFβ
1 in said mixture is greater than 1% of total proteins in said mixture;
wherein the quantity of said BMP-2 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
wherein the quantity of said BMP-3 in said mixture is from about 0.1% to about 15% of total proteins in said mixture; and
,wherein the quantity of said BMP-7 in said mixture is from about 0.01% to about 10% of total proteins in said mixture. - View Dependent Claims (2, 3, 6, 7, 11, 12, 13, 14, 18, 19, 20, 21)
-
-
4. A product for repair of cartilage lesions, comprising:
-
a. a cartilage repair matrix; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
(i) a bone-derived osteogenic or chondrogenic formulation containing at least one bone morphogenetic protein (BMP); and
,(ii) a TGFβ
protein that is exogenous to said formulation of (i);
wherein the weight ratio of said exogenous TGFβ
protein to total BMP in said mixture of proteins is greater than about 10;
1; and
,wherein said exogenous TGFβ
protein is present in an amount sufficient to increase cartilage induction by said composition over a level of cartilage induction by said bone-derived osteogenic or chondrogenic protein formulation in the absence of said exogenous TGFβ
protein. - View Dependent Claims (5, 8, 9)
-
-
10. A product for repair of cartilage lesions, comprising:
-
a. a cartilage repair matrix; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
(i) a TGFβ
protein; and
,(ii) at least one bone morphogenetic protein (BMP);
wherein the weight ratio of said TGFβ
protein to total BMP in said mixture of proteins is greater than about 10;
1. - View Dependent Claims (15, 16, 17, 22, 24, 25, 38)
-
-
23. A product for repair of cartilage lesions, comprising:
-
a. a cartilage repair matrix suitable for conforming to a defect in cartilage; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
transforming growth factor β
1 (TGFβ
1), bone morphogenetic protein (BMP)-2, BMP-3, and BMP-7;
wherein the quantity of said TGFβ
1 in said mixture is greater than 1% of total proteins in said mixture;
wherein the quantity of said BMP-2 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
wherein the quantity of said BMP-3 in said mixture is at least about 0.38% of total proteins in said mixture; and
,wherein the quantity of said BMP-7 in said mixture is from about 0.01% to about 10% of total proteins in said mixture.
-
-
26. A method for repair of cartilage lesions, comprising implanting and fixing into a cartilage lesion a product comprising:
-
a. a cartilage repair matrix suitable for conforming to a defect in cartilage; and
b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
transforming growth factor β
1 (TGFβ
1), bone morphogenetic protein (BMP)-2, BMP-3, and BMP-7;
wherein the quantity of said TGFβ
1 in said mixture is greater than 1% of total proteins in said mixture;
wherein the quantity of said BMP-2 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
wherein the quantity of said BMP-3 in said mixture is from about 0.1% to about 15% of total proteins in said mixture; and
,wherein the quantity of said BMP-7 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
whereby implanting and fixing said product into said cartilage lesion enhances repair of said defect in cartilage as compared to in the absence of said product. - View Dependent Claims (29, 30, 33, 34, 35, 36, 41)
-
-
27. A method for repair of cartilage lesions, comprising implanting and fixing into a cartilage lesion a product comprising:
-
a. a cartilage repair matrix; and
,b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
(i) a bone-derived osteogenic or chondrogenic formulation of proteins containing at least one bone morphogenetic protein (BMP); and
,(ii) a TGFβ
protein that is exogenous to said formulation of (i);
wherein the weight ratio of said exogenous TGFβ
protein to total BMP in said mixture of proteins is greater than about 10;
1; and
,wherein said exogenous TGFβ
protein is present in an amount sufficient to increase cartilage induction by said composition over a level of cartilage induction by said bone-derived osteogenic or chondrogenic protein formulation in the absence of said exogenous TGFβ
protein;
whereby implanting and fixing said product into said cartilage lesion enhances repair of said defect in cartilage as compared to in the absence of said product. - View Dependent Claims (28, 37)
-
-
31. A method for repair of cartilage lesions, comprising implanting and fixing into a cartilage lesion a product comprising:
-
a. a cartilage repair matrix; and
,b. a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
(i) a TGFβ
protein; and
,(ii) at least one bone morphogenetic protein (BMP);
wherein the weight ratio of said TGFβ
protein to total BMP in said mixture of proteins is greater than about 10;
1;
whereby implanting and fixing said product into said cartilage lesion enhances repair of said defect in cartilage as compared to in the absence of said product. - View Dependent Claims (32)
-
-
39. A method for repair of segmental cartilage lesions, comprising implanting and fixing into a segmental cartilage lesion:
-
a. a first product comprising;
(i) a cartilage repair matrix configured as a sheet; and
(ii) a cartilage-inducing composition contained on or within said matrix comprising a mixture of proteins comprising;
transforming growth factor β
1 (TGFβ
1), bone morphogenetic protein (BMP)-2, BMP-3, and BMP-7;
wherein the quantity of said TGFβ
1 in said mixture is greater than 1% of total proteins in said mixture;
wherein the quantity of said BMP-2 in said mixture is from about 0.01% to about 10% of total proteins in said mixture;
wherein the quantity of said BMP-3 in said mixture is from about 0.1% to about 15% of total proteins in said mixture; and
,wherein the quantity of said BMP-7 in said mixture is from about 0.01% to about 10% of total proteins in said mixture; and
,b. a second product comprising a cartilage repair matrix configured to replace cartilage removed from a segmental lesion;
wherein said second product is implanted into said lesion and wherein said first product is implanted between an edge of said lesion and said second product to provide an interface between said lesion and said second product. - View Dependent Claims (40)
-
-
42. A composition comprising:
-
at least one TGFβ
protein; and
at least one bone morphogenetic protein (BMP);
wherein the weight ratio of said at least one TGFβ
protein to said at least one BMP is a chondrogenesis inducing ratio greater than 10;
1. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80)
-
Specification