Nanostructured separation and analysis devices for biological membranes
First Claim
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1. A nanostructured device comprising:
- a substrate including at least one nanotrough therein; and
a lipid bilayer suspended over said at least one nanotrough.
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Abstract
The present invention provides a nanostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the substrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures.
189 Citations
62 Claims
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1. A nanostructured device comprising:
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a substrate including at least one nanotrough therein; and
a lipid bilayer suspended over said at least one nanotrough. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A nanostructured device comprising:
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a substrate including at least one nanotrough therein; and
at least one lipid bilayer supported in at least one of said at least one nanotroughs so as to allow biomolecules to pass from said at least one lipid bilayer into said at least one respective nanotrough. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A separation method comprising the steps of:
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(a) supporting or suspending a lipid bilayer on a substrate;
wherein said substrate comprises at least one nanostructure and wherein said lipid bilayer comprises at least one membrane associated biomolecule; and
(b) applying a driving force to said lipid bilayer to separate said at least one membrane associate biomolecule from said lipid bilayer and to drive said at least one membrane associated biomolecule within said lipid bilayer into said at least one nanostructure. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method for separating particles comprising:
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(a) providing a substrate and a plurality of structures arranged on said substrate, wherein said structures comprise successive rows of protrusions, said protrusions exhibiting a gradient property across a plane of said substrate and in a direction transverse to the successive rows of protrusions, the protrusions of each row being of substantially the same size across at least a portion of the substrate; and
(b) flowing a plurality of said particles through said structures in said direction transverse to the successive rows of protrusions to effect separation of said plurality of particles based on affinity. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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60. A method for separating particles comprising:
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(a) providing a substrate and a plurality of structures arranged on said substrate, wherein said structures comprise successive rows of protrusions and channels or gaps between the protrusions, the protrusions exhibiting a gradient property across a plane of said substrate and in a direction transverse to the successive rows of protrusions, the channels or gaps exhibiting a gradient property across a plane of said substrate and in a direction transverse to the successive rows of protrusions, the protrusions of each row being of substantially the same size across at least a portion of the substrate, the channels or gaps of each row being of substantially the same size across at least a portion of the substrate, protrusions in at least one row being of substantially the same size as protrusions in at least one respectively adjacent row, channels or gaps in at least one row being of substantially the same size as channels or gaps in at least one respectively adjacent row; and
(b) flowing a plurality of said particles through said structures in said direction transverse to the successive rows of protrusions to effect separation of said plurality of particles based on affinity. - View Dependent Claims (61, 62)
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Specification