Nanostructured separation and analysis devices for biological membranes
First Claim
1. A nanostructured device comprising:
- a substrate including at least one nanotrough therein; and
a lipid bilayer suspended over said at least one nanotrough.
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Abstract
The present invention provides a nonostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the subtrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures.A fluidic device for separating particles according to size is provided including a fluidic channel, and a matrix comprising a plurality of protrusions within the fluidic channel, wherein the device provides a driving force to the particles being separated through the fluidic channel; and wherein a flow of the driving force from between the protrusions is divided unequally into a major flow component and a minor flow component, each component flowing between subsequent protrusions in the matrix, such that the average direction of the major flow component is not parallel to the average direction of the driving force, and, when particles are introduced into the matrix, particles having a size less than a predetermined critical size are transported generally in the average direction of the driving force, and particles having a size at least that of the critical size are transported generally in the average direction of the major flow component, thereby separating the particles according to size. Methods for separating particles including steps of separation based on size and affinity are also provided.
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Citations
56 Claims
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1. A nanostructured device comprising:
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a substrate including at least one nanotrough therein; and
a lipid bilayer suspended over said at least one nanotrough. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A nanostructured device comprising:
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a substrate including at least one nanotrough therein; and
at least one lipid bilayer supported in at least one of said at least one nanotroughs so as to allow biomolecules to pass from said at least one lipid bilayer into said at least one respective nanotrough. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A separation method comprising the steps of:
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(a) supporting or suspending a lipid bilayer on a substrate;
wherein said substrate comprises at least one nanostructure and wherein said lipid bilayer comprises at least one membrane associated biomolecule; and
(b) applying a driving force to said lipid bilayer to separate said at least one membrane associate biomolecule from said lipid bilayer and to drive said at least one membrane associated biomolecule within said lipid bilayer into said at least one nanostructure. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method for separating particles comprising:
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(a) providing a substrate and an array of structures arranged on said substrate, wherein said structures comprise protrusions fixed to said substrate in a predetermined pattern having a gradient property in a given direction across a plane of said substrate;
(b) a first separation step comprising separating said particles by size in two dimensions by flowing said particles through said array of structures in said given direction, the separating of said particles occurring at least in part by virtue of said pattern; and
(c) a second separation step comprising separating said particles by affinity. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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Specification