Nanostructured separation and analysis devices for biological membranes
First Claim
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1. A nanostructured device comprising:
- a substrate including an upper face or surface and at least one elongate nanotrough therein extending downwardly into said substrate from said upper face or surface, said at least one elongate nanotrough having continuous or uninterrupted opposing walls along at least a substantial length thereof; and
a separation and analysis platform in the form of a singular continuous lipid bilayer in part disposed on said upper face or surface and in part suspended over or supported in said at least one elongate nanotrough, said lipid bilayer having sufficient fluidity to allow for mobility of biomolecules embedded within said lipid bilayer.
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Abstract
The present invention provides a nanostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the substrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures.
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Citations
55 Claims
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1. A nanostructured device comprising:
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a substrate including an upper face or surface and at least one elongate nanotrough therein extending downwardly into said substrate from said upper face or surface, said at least one elongate nanotrough having continuous or uninterrupted opposing walls along at least a substantial length thereof; and
a separation and analysis platform in the form of a singular continuous lipid bilayer in part disposed on said upper face or surface and in part suspended over or supported in said at least one elongate nanotrough, said lipid bilayer having sufficient fluidity to allow for mobility of biomolecules embedded within said lipid bilayer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 48, 49)
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11. A nanostructured device comprising:
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a substrate including a nanostructured matrix with an upper face or surface and at least one elongate nanotrough thereinextending downwardly into said substrate from said upper face or surface; and
a separation and analysis platform in the form of at least one singular continuous lipid bilayer in part disposed on said upper face or surface and in part supported in or suspended over at least one ofsaid at least one elongate nanotroughs so as to allow biomolecules to pass from said at least one lipid bilayer into said at least one respective nanotrough, said lipid bilayer having sufficient fluidity to allow for mobility of biomolecules embedded within said device so that said biomolecules are moveable to effectuate a separation of said biomolecules within said lipid bilayer. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 50, 51)
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20. A separation method comprising the steps of:
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(a) supporting or suspending a separation and analysis platform in the form of a lipid bilayer on a substrate;
wherein said substrate comprises at least one nanostructureelongate nanotrough, said nanotrough having continuous or uninterrupted opposing walls along at least a substantial length thereof, and wherein said lipid bilayer comprises at least onehas sufficient fluidity to allow for mobility of membrane associated biomolecules embedded within said separation and analysis platform; and
(b) applying a driving force to said lipid bilayer to separate said at least onemembrane associated biomolecules from said lipid bilayereach other within said separation and analysis platform and to drive said at least onemembrane associated biomolecules within and parallel to or in a plane of said lipid bilayer intoseparation and analysis platform by virtue of the fluidity of said lipid bilayer, and guided by said at least one nanostructurenanotrough. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 52, 53)
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38. A separation method comprising:
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providing a nanostructured device comprising (i) a substrate including a nanostructured matrix with at least one elongate nanotrough therein, (ii) a separation and analysis platform in the form of a lipid bilayer suspended over or supported in said nanostructured matrix and said at least one elongate nanotrough, said lipid bilayer being derived from cell organelles, and (iii) a plurality of membrane-associated biomolecules occurring within said device and extending in said at least one elongate nanotrough, said lipid bilayer having sufficient fluidity to allow for mobility of biomolecules embedded within said device; and
applying a driving force to said lipid bilayer to drive said biomolecules within said device, by virtue of the fluidity of said lipid bilayer, and in said at least one elongate nanotrough, to separate said biomolecules from each other in said device under a screening action of said nanostructured matrix. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 54, 55)
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Specification