Apparatus for chemical amplification based on fluid partitioning in an immiscible liquid
DCFirst Claim
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1. An apparatus for nucleic acid amplification of a sample, comprising:
- means for partitioning said sample into partitioned sections, wherein said means for partitioning said sample into partitioned sections comprises an injection orifice, andmeans for performing PCR on said partitioned sections of said sample.
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Abstract
A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
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Citations
58 Claims
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1. An apparatus for nucleic acid amplification of a sample, comprising:
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means for partitioning said sample into partitioned sections, wherein said means for partitioning said sample into partitioned sections comprises an injection orifice, and means for performing PCR on said partitioned sections of said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of nucleic acid amplification of a sample, comprising the steps of:
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partitioning said sample into partitioned sections, wherein said step of partitioning said sample into partitioned sections comprises flowing said sample through an injection orifice, and subjecting said partitioned sections of said sample to PCR. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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- 22. A nucleic acid amplification apparatus comprising a microdroplet generator comprising an orifice, wherein said orifice connects a sample flow pathway to a channel or tube comprising an immiscible fluid, and wherein said channel or tube passes through a heating element.
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34. A nucleic acid amplification apparatus comprising:
a microdroplet generator comprising an orifice wherein said orifice connects a sample flow pathway to a channel or tube comprising an immiscible fluid, wherein said channel or tube passes through a heating element; and
wherein said apparatus further comprises a detector capable of detecting microdroplets in said immiscible fluid.- View Dependent Claims (35)
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36. A method for nucleic acid amplification comprising:
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producing microdroplets within an immiscible fluid in a channel or tube;
wherein the microdroplets comprise nucleic acids and components for performing nucleic acid amplification;moving the microdroplets through the channel or tube; and thermal cycling the microdroplets in the channel or tube to amplify the nucleic acids. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
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47. A method comprising:
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diluting a sample comprising a plurality of DNA targets and PCR reagents; partitioning the sample into microdroplets in an immiscible fluid in a tube or channel of a microfluidic device, wherein a plurality of microdroplets containing a single template of the target DNA are formed; and
amplifying the target DNA in the microdroplets by heating and cooling such that a plurality of single templates within the microdroplets are amplified.
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48. A method comprising:
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a. performing PCR on a microdroplet suspended in an immiscible fluid in a microchannel, wherein said PCR comprises a plurality of cycles; b. passing said microdroplet through said microchannel past a detector; and c. detecting a PCR amplification product in said microdroplet. - View Dependent Claims (49, 50, 51)
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52. A nucleic acid amplification apparatus comprising:
- a microdroplet generator comprising an orifice wherein said orifice connects a sample flow pathway to a channel or tube comprising an immiscible fluid, wherein said channel or tube passes through a heating element; and
wherein said apparatus further comprises a detector capable of detecting microdroplets in said immiscible fluid and a pump for moving said microdroplets through the channel or tube. - View Dependent Claims (53, 54, 55, 56, 57, 58)
- a microdroplet generator comprising an orifice wherein said orifice connects a sample flow pathway to a channel or tube comprising an immiscible fluid, wherein said channel or tube passes through a heating element; and
Specification