Method of determining an analyte concentration of a fluid

• US 10,010,279 B2
• Filed: 04/17/2017
• Issued: 07/03/2018
• Est. Priority Date: 12/27/2005
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Inventors: Brenneman, Allen J, Rebec, Mihailo V.
• Primary Examiner(s): WEARE, MEREDITH H
• Application Number:
  US15/488,886
• Publication Number:
  US 20170273604A1
• Time in Patent Office: 442 Days
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1468 using chemical or electrochemical methods, e.g. by polarographic means A61B5/1486 takes precedence
• CPC Class Codes: A61B 5/1477 non-invasive
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10T 29/49124 On flat or curved insulated base, e.g., printed circuit, etc.

Abstract

An electrochemical sensor system is adapted to assist in determining an analyte concentration of a fluid. The electrochemical sensor system comprises a substrate, conductive material and a hydrogel or liquid. The substrate having porosity therethrough. The conductive material includes at least one electrode. The at least one electrode is coupled to the substrate. The at least one electrode has a first surface and an opposing second surface. The hydrogel or liquid is adapted to assist in carrying the analyte of the fluid to the first and second surfaces of the at least one electrode.

Claims

1. A method of determining an analyte concentration of a fluid, the method comprising the acts of:

providing an electrochemical sensor system including;

a substrate having porosity including at least one aperture extending through the substrate,

an enzyme coupled to the substrate, the enzyme being configured to chemically react with the analyte in the fluid,

at least one conductive electrode coupled to the substrate, each electrode of the at least one electrode having a first surface and a second surface on the opposite side of the first surface, and

a hydrogel having a first layer and a second layer, the hydrogel extending through the at least one aperture and contacting the first surface and the second surface of the at least one electrode and the substrate, the hydrogel being configured to carry the analyte of the fluid into contact with both the first surface and the second surface of the at least one electrode, with the at least one electrode and the substrate being sandwiched between the first layer and the second layer of the hydrogel;

placing the electrochemical sensor system on the skin; and

determining the analyte concentration of the fluid.

2. The method of claim 1, wherein the analyte is glucose.

3. The method of claim 1, wherein the substrate is a screen, scrim, woven material or solid material with apertures.

4. The method of claim 1, wherein the porosity of the substrate includes a plurality of apertures formed therethrough.

5. The method of claim 1, wherein the at least one electrode is a plurality of electrodes, the plurality of electrodes including a working electrode and a counter electrode.

6. The method of claim 1, wherein the electrochemical sensor system uses a liquid.

7. The method of claim 1, wherein the fluid is an intercellular fluid.

8. The method of claim 7, wherein the fluid is interstitial fluid.