Systems, models and methods for identifying and evaluating skin-active agents effective for treating dandruff/seborrheic dermatitis
First Claim
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1. A method for constructing a data architecture for use in identifying connections between perturbagens and genes associated with dandruff, and preparing a dandruff care composition comprising:
- (a) providing a gene expression profile for a control human epidermal keratinocyte cell;
(b) generating a gene expression profile for a human epidermal keratinocyte cell exposed to at least one perturbagen, by extracting a biological sample from the treated cell and subjecting the biological sample to microarray analysis via a microarray scanner wherein generating the gene expression profile in at least one of (a) and (b) comprisesi. isolating RNA from the human epidermal keratinocyte cell, andii. creating cDNA from the isolated RNA;
(c) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (a) and (b);
(d) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes;
(e) storing the ordered list as a keratinocyte instance on at least one computer readable medium; and
(f) constructing a data architecture of stored keratinocyte instances by repeating (a) through (e), wherein the at least one perturbagen of step (a) is different qualitatively or quantitatively for each keratinocyte instance(g) querying the data architectures of stored keratinocyte instances with at least one dandruff gene expression signature, wherein querying comprises comparing the at least one dandruff gene expression signature to each stored keratinocyte instance, wherein the dandruff gene expression represents genes differentially expressed in association with dandruff;
(h) assigning a connectivity score to each of the instances; and
(i) preparing a dandruff care composition comparing at least one perturbagen, wherein the connectivity score of the instance associated with the at least one perturbagen has a negative correlation.
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Abstract
Methods and systems for determining functional relationships between a skin-active agent and a skin condition of interest, and methods and systems for identifying cosmetic agents effective for treatment of dandruff, as well as the use of agents identified by such methods and systems for the preparation of cosmetic compositions, personal care products, or both are provided. Methods for developing in vitro models of skin disease and models for specific skin diseases are also provided.
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22 Claims
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1. A method for constructing a data architecture for use in identifying connections between perturbagens and genes associated with dandruff, and preparing a dandruff care composition comprising:
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(a) providing a gene expression profile for a control human epidermal keratinocyte cell; (b) generating a gene expression profile for a human epidermal keratinocyte cell exposed to at least one perturbagen, by extracting a biological sample from the treated cell and subjecting the biological sample to microarray analysis via a microarray scanner wherein generating the gene expression profile in at least one of (a) and (b) comprises i. isolating RNA from the human epidermal keratinocyte cell, and ii. creating cDNA from the isolated RNA; (c) identifying genes differentially expressed in response to the at least one perturbagen by comparing the gene expression profiles of (a) and (b); (d) creating an ordered list comprising identifiers representing the differentially expressed genes, wherein the identifiers are ordered according to the differential expression of the genes; (e) storing the ordered list as a keratinocyte instance on at least one computer readable medium; and (f) constructing a data architecture of stored keratinocyte instances by repeating (a) through (e), wherein the at least one perturbagen of step (a) is different qualitatively or quantitatively for each keratinocyte instance (g) querying the data architectures of stored keratinocyte instances with at least one dandruff gene expression signature, wherein querying comprises comparing the at least one dandruff gene expression signature to each stored keratinocyte instance, wherein the dandruff gene expression represents genes differentially expressed in association with dandruff; (h) assigning a connectivity score to each of the instances; and (i) preparing a dandruff care composition comparing at least one perturbagen, wherein the connectivity score of the instance associated with the at least one perturbagen has a negative correlation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification