Hand-held laser biosensor

• US 10,132,752 B2
• Filed: 09/21/2017
• Issued: 11/20/2018
• Est. Priority Date: 01/27/2017
• Status: Active Grant

• Current Assignee: the united states of america as represented by the secretary of the navy
• Original Assignee: the united states of america as represented by the secretary of the navy
• Inventors: Ng, Kin Chiu, Sanyal, Subrata
• Primary Examiner(s): Nur, Abdullahi
• Application Number:
  US15/712,122
• Publication Number:
  US 20180217061A1
• Time in Patent Office: 425 Days
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 2015/0046 in gas, e.g. smoke
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held

Abstract

A hand-held biosensor including a housing having a lower handgrip portion and an upper target portion extending substantially perpendicular to the lower handgrip portion. An electromagnetic radiation emitter is received within the lower handgrip portion for emitting a laser beam toward a band pass filter received within the upper target portion. The band pass filter is configured to reflect the laser beam toward a target, and to permit passage therethrough of fluorescence emissions from the target. A photo-detector is received within the upper target portion of the housing and is configured to receive the fluorescence emissions from the target. A signal display is configured to provide an indication of fluorescence spectral data from the photo-detector. An adapter may be releasably coupled to the upper target portion of the housing for receiving atmospheric air.

Claims

1. A hand-held biosensor comprising:

an electromagnetic radiation emitter configured to emit a laser beam in a first direction along a first longitudinal axis, the laser beam having a wavelength between 260 nm and 290 nm;

a first band pass filter configured to reflect the laser beam in a second direction along a second longitudinal axis substantially perpendicular to the first longitudinal axis toward a target, the first band pass filter configured to permit passage therethrough of fluorescence emissions from the target in a third direction along the second longitudinal axis opposite the second direction;

a photo-detector configured to receive the fluorescence emissions;

a processor in electrical communication with the photo-detector to receive fluorescence spectral data from the photo-detector;

a signal display in electrical communication with the processor and configured to provide an indication of the fluorescence spectral data; and

a first power supply in electrical communication with the electromagnetic radiation emitter;

a housing including a lower handgrip portion extending between a lower end and an upper end along the first longitudinal axis, and an upper target portion extending between a proximal end and a distal end along the second longitudinal axis, the electromagnetic radiation emitter received within the lower handgrip portion, and the photo-detector received within the upper target portion an air sample concentrator configured to be coupled to the distal end of the upper target portion of the housing, the air sample concentrator including an inlet for receiving ambient air, an outlet for coupling to a suction pump, and a filter to collect air particlesand biomolecules defining the target.

2. The hand-held biosensor of claim 1, wherein the electromagnetic radiation emitter comprises a laser diode array and a laser driver operably coupled to the laser diode array.

3. The hand-held biosensor of claim 1, further comprising at least one lens received within the target portion of the housing and defining a focal length for the laser beam to engage the target, the target portion of the housing including a first portion telescopingly coupled to a second portion for moving the at least one lens and thereby adjusting the focal length.

4. The hand-held biosensor of claim 1, further comprising a second band pass filter supported at the distal end of the upper target portion of the housing, the second band pass filter restricting passage to electromagnetic radiation having a wavelength of between approximately 250 nm and approximately 400 nm.

5. The hand-held biosensor of claim 1, further comprising a second power supply in electrical communication with the signal display, the second power supply including a battery supported by the target portion of the housing, and the signal display including a liquid crystal display supported by the target portion of the housing.

6. The hand-held biosensor of claim 1, wherein the filter is formedof a material that when impacted with a laser beam having a wavelength between approximately 260 nm and 290 nm, the filter will not interfere spectrally with fluorescence detection of approximately 345 nm wavelength.

7. The hand-held biosensor of claim 6, further comprising a sample concentrator coupled to the distal end of the upper target portion of the housing.

8. A hand-held biosensor comprising:

a housing including a lower handgrip portion extending between a lower end and an upper end along a first longitudinal axis, and an upper target portion extending between a proximal end and a distal end along a second longitudinal axis substantially perpendicular to the first longitudinal axis;

an electromagnetic radiation emitter received within the lower handgrip portion and configured to emit a laser beam in a first direction along the first longitudinal axis, the laser beam having a wavelength between 260 nm and 290 nm;

a first band pass filter configured to reflect the laser beam in a second direction along the second longitudinal axis substantially perpendicular to the first longitudinal axis toward a target, the first band pass filter configured to permit passage therethrough of fluorescence emissions from the target in a third direction along the second longitudinal axis opposite the second direction;

a photo-detector received within the upper target portion of the housing and configured to receive the fluorescence emissions;

a processor in electrical communication with the photo-detector to receive fluorescence spectral data from the photo-detector;

a signal display in electrical communication with the processor and configured to provide an indication of the fluorescence spectral data;

a first power supply in electrical communication with the electromagnetic radiationemitter; and

an adapter releasably coupled to the distal end of the upper target portion of the housing for collecting ambient air, wherein the adapter comprises an air sample concentrator configured to be coupled to the distal end of the upper target portion of the housing, the air sample concentrator including an inlet for receiving ambient air, an outlet for coupling to a suction pump, and a filter to collect air particles and biomolecules.

9. The hand-held biosensor of claim 8, wherein the electromagnetic radiation emitter comprises a laser diode array and a laser driver.

10. The hand-held biosensor of claim 8, further comprising at least one lens received within the target portion of the housing and defining a focal length for the laser beam to engage the target, the target portion of the housing including a first portion telescopingly coupled to a second portion for moving the at least one lens and thereby adjusting the focal length.

11. The hand-held biosensor of claim 8, further comprising a second band pass filter supported at the distal end of the upper target portion of the housing, the second band pass filter restricting passage to electromagnetic radiation having a wavelength of between approximately 250 nm and approximately 400 nm.

12. The hand-held biosensor of claim 8, further comprising a second power supply in electrical communication with the signal display, the second power supply including a battery supported by the target portion of the housing, and the signal display including a liquid crystal display supported by the target portion of the housing.

13. The hand-held biosensor of claim 8, wherein the filter is formed of a material that, when illuminated with a laser beam of approximately 275 nm wavelength, will not interfere spectrally with fluorescence detection of approximately 345 nm wavelength.

14. The hand-held biosensor of claim 8, wherein the adapter comprises a continuous sample flow adaptor configured to be coupled to the distal end of the upper target portion of the housing, the continuous sample flow adaptor including an inlet for receiving ambient air, an outlet for coupling to a suction pump, a protective fused silica-plate, and a concave mirror aligned with the second longitudinal axis.

15. A hand-held biosensor comprising:

a housing including a lower handgrip portion extending between a lower end and an upper end along a first longitudinal axis, and an upper target portion extending between a proximal end and a distal end along a second longitudinal axis substantially perpendicular to the first longitudinal axis;

an electromagnetic radiation emitter received within the lower handgrip portion and configured to emit a laser beam in a first direction along a first longitudinal axis, the laser beam having a wavelength between 260 nm and 290 nm;

a first band pass filter configured to reflect the laser beam in a second direction along a second longitudinal axis substantially perpendicular to the first longitudinal axis toward a target, the first band pass filter configured to permit passage therethrough of fluorescence emissions from the target in a third direction along the second longitudinalaxis opposite the second direction;

a photo-detector received within the upper target portion of the housing adjacent the proximal end and configured to receive the fluorescence emissions;

a second band pass filter supported adjacent the distal end of the upper target portion, the second band pass filter covering the spectral region of between approximately 250 nm and approximately 400 nm;

at least one lens received within the target portion of the housing and defining a focal length for the laser beam to engage the target, the target portion of the housing including a first portion telescopingly coupled to a second portion for moving the at least one lens and thereby adjusting the focal length;

a processor in electrical communication with the photo-detector to receive fluorescence spectral data from the photo-detector;

a signal display in electrical communication with the processor and configured to provide an indication of the fluorescence spectral data;

a first power supply in electrical communication with the electromagnetic radiation emitter;

a second power supply in electrical communication with the signal display, the second power supply including a battery supported by the target portion of the housing, and the signal display including a liquid crystal display supported by the target portion of the housing; and

an adapter releasably coupled to the distal end of the upper target portion of the housing for collecting ambient air;

a continuous flow air sample concentrator configured to be coupled to the distal end of the upper target portion of the housing, the continuous flow air sample concentrator including an inlet for receiving ambient air, an outlet for coupling to a suction pump, and a filter to collect air particles and biomolecules.

16. The hand-held biosensor of claim 15, wherein the filter is formed of a material that when illuminated with a laser beam of approximately 275 nm wavelength, it will not interfere spectrally with fluorescence detection of approximately 345 nmwavelength.

17. The hand-held biosensor of claim 15, further comprising of a sampling hose connected to the inlet of the continuous flow air sample concentrator for receiving ambient air.

18. A hand-held biosensor comprising:

a housing including a lower handgrip portion extending between a lower end and an upper end along a first longitudinal axis, and an upper target portion extending between a proximal end and a distal end along a second longitudinal axis substantially perpendicular to the first longitudinal axis;

an electromagnetic radiation emitter received within the lower handgrip portion and configured to emit a laser beam in a first direction along a first longitudinal axis, the laser beam having a wavelength between 260 nm and 290 nm;

a first band pass filter configured to reflect the laser beam in a second direction along a second longitudinal axis substantially perpendicular to the first longitudinal axis toward a target, the first band pass filter configured to permit passage therethrough of fluorescence emissions from the target in a third direction along the second longitudinal axis opposite the second direction;

a photo-detector received within the upper target portion of the housing adjacent the proximal end and configured to receive the fluorescence emissions;

a second band pass filter supported adjacent the distal end of the upper target portion, the second band pass filter covering the spectral region of between approximately 250 nm andapproximately 400 nm;

at least one lens received within the target portion of the housing and defining a focal length for the laser beam to engage the target, the target portion of the housing including a first portion telescopingly coupled to a second portion for moving the at least one lens and thereby adjusting the focal length;

a processor in electrical communication with the photo-detector to receive fluorescence spectral data from the photo-detector;

a signal display in electrical communication with the processor and configured to provide an indication of the fluorescence spectral data;

a first power supply in electrical communication with the electromagnetic radiation emitter;

a second power supply in electrical communication with the signal display, the second power supply including a battery supported by the target portion of the housing, and the signal display including a liquid crystal display supported by the target portion of the housing; and

an adapter releasably coupled to the distal end of the upper target portion of the housing for collecting ambient air

a continuous sample flow adaptor configured to be coupled to the distal end of the upper target portion of the housing, the continuous sample flow adaptor including an inlet for receiving ambient air, an outlet for coupling to a suction pump, and a concave mirror aligned with the second longitudinal axis.

19. The hand-held biosensor of claim 18, further comprising a sampling hose connected to the inlet of the continuous sample flow adaptor for receiving ambient air.