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Methods for the detection of analytes in small-volume blood samples

  • US 10,156,579 B2
  • Filed: 02/26/2016
  • Issued: 12/18/2018
  • Est. Priority Date: 11/14/2006
  • Status: Active Grant
First Claim
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1. A method of detecting an analyte in a small-volume blood sample obtained from a subject, comprising:

  • a) obtaining a sample of blood from a subject by lancing or pipetting, said blood sample having a volume of less than about 500 μ

    L;

    b) pre-treating the blood sample by one or more of diluting, mixing, drying, evaporating, centrifuging, and extracting;

    c) introducing the blood sample into a fluidic device comprising a sample collection unit and an assay assembly, said assay assembly comprising reactants that are capable of reacting with said analyte;

    d) allowing the blood sample to react with said reactants contained within said assay assembly to yield a colored product having an absorbance spectrum corresponding to at least one wavelength from a light source located within said fluidic device;

    e) transmitting the at least one wavelength to the fluidic device from said light source, wherein said light source comprises a luminescent paint; and

    f) detecting a change in absorption of light of the at least one wavelength transmitted to the fluidic device, wherein said change in absorption is indicative of the presence of the analyte in the blood sample.

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