Cell evaluation apparatus and method to search for an area to be observed in cardiomyocytes

• US 10,157,459 B2
• Filed: 07/10/2014
• Issued: 12/18/2018
• Est. Priority Date: 07/19/2013
• Status: Expired due to Fees

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Kunihiro, Takeshi, Hayakawa, Tomohiro, Matsui, Eriko, Kobayashi, Seiji
• Primary Examiner(s): MOYER, ANDREW M
• Application Number:
  US14/904,861
• Publication Number:
  US 20160163044A1
• Time in Patent Office: 1,622 Days
• Field of Search: 382133, 382134
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells
• CPC Class Codes: G01N 15/10 Investigating individual particles
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G06T 2207/20021 Dividing image into blocks, subimages or windows
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 2207/30048 Heart; Cardiac
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06T 7/0016 involving temporal comparison
• CPC Class Codes: G06T 7/246 using feature-based methods, e.g. the tracking of corners or segments
• CPC Class Codes: G06T 7/32 using correlation-based methods
• CPC Class Codes: G06V 20/693 Acquisition

Abstract

The present technology relates to a cell evaluation apparatus and method, and a program that are capable of easily searching for an area to be observed within cardiomyocytes without requiring special preparation.

The cell evaluation apparatus includes: a motion detecting section configured to detect motion of cultured cardiomyocytes for each of partial areas of a plurality of observation fields on the cultured cardiomyocytes; a motion amount calculating section configured to calculate a motion amount of the detected motion; a pulsation information calculating section configured to calculate pulsation information on a characteristic amount of pulsations of the cultured cardiomyocytes on the basis of the calculated motion amount; an evaluation value calculating section configured to calculate an evaluation value corresponding to the plurality of observation fields, calculated on the basis of the pulsation information; and a field determining section configured to determine an observation field to be observed on the cultured cardiomyocytes, on the basis of the evaluation value.

Claims

1. A cell evaluation apparatus, comprising:

circuitry configured to;

detect motion of cultured cardiomyocytes for each observation field of a plurality of observation fields that correspond to a plurality of partial areas on the cultured cardiomyocytes;

calculate a motion amount of the detected motion;

calculate pulsation information on a characteristic amount of pulsations of the cultured cardiomyocytes based on the calculated motion amount,

wherein the pulsation information, is a pulsation area of the cultured cardiomyocytes within an observation field of the plurality of observation fields, calculated based on comparison of an added value of pieces of block unit motion amount data corresponding to respective blocks with a threshold value,

wherein each respective block of the respective blocks correspond to a respective area of the plurality of partial areas on the cultured cardiomyocytes;

calculate an evaluation value corresponding to the plurality of observation fields based on the pulsation information; and

determine the observation field observed on the cultured cardiomyocytes based on the evaluation value.

2. The cell evaluation apparatus according to claim 1, wherein the circuitry is further configured to:

detect the motion for each observation field of the plurality of observation fields corresponding to the plurality of partial areas in each frame of a plurality of frames of a moving image of an observation area, and

generate, wave shape information that represents the pulsations of the cultured cardiomyocytes of each partial area of the plurality of partial areas based on the motion amount for each observation field of the plurality of observation fields of the plurality of frames of the moving image.

3. The cell evaluation apparatus according to claim 2, wherein the pulsation information is a number of pulsations of the cultured cardiomyocytes within the observation field within a unit time, and wherein the pulsation information is calculated based on the wave shape information.

4. The cell evaluation apparatus according to claim 2, wherein the pulsation information is one of a contraction time or a relaxation time of the pulsations of the cultured cardiomyocytes within the observation field, and wherein the pulsation information is calculated based on the wave shape information.

5. The cell evaluation apparatus according to claim 2, wherein the pulsation information is one of a contraction speed or a relaxation speed of the pulsations of the cultured cardiomyocytes within the observation field, and wherein the pulsation information is calculated based on the wave shape information.

6. The cell evaluation apparatus according to claim 2, wherein the pulsation information is a correlation coefficient of a wave shape of the pulsations between the plurality of partial areas of the cultured cardiomyocytes within the observation field, and wherein the pulsation information is calculated based on the wave shape information.

7. The cell evaluation apparatus according to claim 2, wherein the pulsation information is a propagation speed and a propagation direction of the pulsations of the cultured cardiomyocytes within the observation field, and wherein the pulsation information is calculated based on the wave shape information.

8. The cell evaluation apparatus according to claim 1, wherein the circuitry is further configured to:

calculate the evaluation value that corresponds to each observation field of the plurality of observation fields, before medication and after the medication on the cultured cardiomyocytes, and

determine the observation field based on selection of a subset of observation fields from the plurality of observation fields based on a difference between the evaluation value before the medication and the evaluation value after the medication on the cultured cardiomyocytes.

9. A cell evaluation method, comprising:

in a cell evaluation apparatus comprising circuitry;

detecting, by the circuitry, motion of cultured cardiomyocytes for each observation field of a plurality of observation fields that correspond to a plurality of partial areas on the cultured cardiomyocytes;

calculating, by the circuitry, a motion amount of the detected motion;

calculating, by the circuitry, pulsation information on a characteristic amount of pulsations of the cultured cardiomyocytes based on the calculated motion amount,

wherein the pulsation information, is a pulsation area of the cultured cardiomyocytes within an observation field of the plurality of observation fields, calculated based on comparison of an added value of pieces of block unit motion amount data corresponding to respective blocks with a threshold value,

wherein each respective block of the respective blocks correspond to a respective area of the plurality of partial areas on the cultured cardiomyocytes;

calculating, by the circuitry, an evaluation value corresponding to the plurality of observation fields based on the pulsation information; and

determining, by the circuitry, the observation field observed on the cultured cardiomyocytes based on the evaluation value.

10. A non-transitory computer-readable medium having stored thereon, computer-executable instructions for causing a cell evaluation apparatus to execute operations, the operations comprising:

detecting motion of cultured cardiomyocytes for each observation field of a plurality of observation fields that correspond to a plurality of partial areas on the cultured cardiomyocytes;

calculating a motion amount of the detected motion;

calculating pulsation information on a characteristic amount of pulsations of the cultured cardiomyocytes based on the calculated motion amount,

wherein the pulsation information, is a pulsation area of the cultured cardiomyocytes within an observation field of the plurality of observation fields, calculated based on comparison of an added value of pieces of block unit motion amount data corresponding to respective blocks with a threshold value,

wherein each respective block of the respective blocks correspond to a respective area of the plurality of partial areas on the cultured cardiomyocytes;

calculating an evaluation value corresponding to the plurality of observation fields based on the pulsation information; and

determining the observation field observed on the cultured cardiomyocytes based on the evaluation value.