OX40L fusion protein for the immunotherapy of tumors of veterinary animals

• US 10,196,435 B2
• Filed: 11/17/2014
• Issued: 02/05/2019
• Est. Priority Date: 11/18/2013
• Status: Active Grant

• Current Assignee: University of Southern California
• Original Assignee: University of Southern California
• Inventors: Epstein, Alan L., Hu, Peisheng, Ohlfest, John
• Primary Examiner(s): Dent, Alana Harris
• Application Number:
  US14/543,714
• Publication Number:
  US 20150191525A1
• Time in Patent Office: 1,541 Days
• Field of Search: None
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 2039/55516 Proteins; Peptides
• CPC Class Codes: A61K 2039/55561 CpG containing adjuvants; Oligonucleotide containing adjuvants
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/0011 Cancer antigens
• CPC Class Codes: A61K 39/001111 Immunoglobulin superfamily
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: C07K 14/575 Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C07K14/665, e.g. corticotropin C07K14/695
• CPC Class Codes: C07K 14/70575 NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154 NGF C07K14/48, TNF C07K14/525
• CPC Class Codes: C07K 14/70578 NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95 NGF-receptor C07K14/71, TNF-receptor C07K14/7151
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

Provided is an isolated recombinant polypeptide comprising an immunoglobulin domain and a canine OX40L extracellular domain polypeptide fragment or a biological equivalent thereof and compositions comprising: an isolated recombinant polypeptide comprising an immunoglobulin domain and a canine OX40L extracellular domain polypeptide fragment; and a pharmaceutically acceptable carrier. Also provided are methods for treating or ameliorating the symptoms of cancer in a canine comprising administering an effective amount of the isolated recombinant polypeptide of the disclosure and/or a composition of the disclosure to a canine in need thereof.

Claims

1. An isolated recombinant polypeptide SEQ ID NO:

3.

2. A composition comprising:

a) the isolated recombinant polypeptide of claim 1; and

b) a pharmaceutically acceptable carrier.

3. The composition of claim 2, further comprising a vaccine comprising a cancer cell lysate or immune cells.

4. The composition of claim 2, further comprising an adjuvant.

5. The composition of claim 4, wherein the adjuvant is an oligonucleotide comprising one or more immunostimulatory sequence motifs, the motif comprising at least one unmethylated CG dinucleotide.

6. The composition of claim 5, wherein the oligonucleotide contains multiple immunostimulatory sequence motifs.

7. The composition of claim 6, wherein the immunostimulatory sequence motif comprises one or both of SEQ ID NO:

4 or SEQ ID NO;

5.

8. A kit comprising the composition of claim 2 and instructions for use.