Endophytes, associated compositions, and methods of use thereof

• US 10,212,911 B2
• Filed: 06/26/2015
• Issued: 02/26/2019
• Est. Priority Date: 06/26/2014
• Status: Active Grant

• Current Assignee: Indigo Agriculture, Inc.
• Original Assignee: Indigo Agriculture, Inc.
• Inventors: von Maltzahn, Geoffrey, Sadowski, Craig, Lyford, Jeffrey, Ambrose, Karen V., Zhang, Xuecheng, Flavell, Richard Bailey, Toledo, Gerardo V., Leff, Jonathan W., Samayoa, Phillip, Marquez, Luis Miguel, Johnston, David Morris, Djonovic, Slavica, Millet, Yves Alain
• Primary Examiner(s): Kruse, David H
• Application Number:
  US14/752,829
• Publication Number:
  US 20150373993A1
• Time in Patent Office: 1,341 Days
• CPC Class Codes: A01H 17/00 Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza lichens A01H15/00
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: C05F 11/08 Organic fertilisers containing added bacterial cultures, mycelia or the like

Abstract

Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

Claims

1. A synthetic combination comprising a purified microbial population in association with a plurality of seeds or seedlings of an agricultural plant, wherein the purified microbial population comprises a first endophyte capable of production of acetoin, wherein the first endophyte is of the genus Cladosporium and comprises an ITS rRNA nucleic acid sequence comprising SEQ ID NO:

3694, and wherein the first endophyte is present in the synthetic combination in an amount effective to provide a benefit to the seeds or seedlings or the plants derived from the seeds or seedlings.

2. The synthetic combination of claim 1, wherein the first endophyte is localized on the surface of the seeds or seedlings.

3. The synthetic combination of claim 1, wherein the first endophyte is obtained from a plant species other than the seeds or seedlings of the synthetic combination.

4. The synthetic combination of claim 1, wherein the benefit is selected from the group consisting of increased root biomass, increased root length, increased height, increased shoot length, increased leaf number, increased water use efficiency, increased overall biomass, increase grain yield, increased photosynthesis rate, increased tolerance to drought, increased heat tolerance, increased salt tolerance, increased resistance to nematode stress, increased resistance to a fungal pathogen, increased resistance to a bacterial pathogen, increased resistance to a viral pathogen, a detectable modulation in the level of a metabolite, and a detectable modulation in the proteome relative to a reference plant.

5. The synthetic combination of claim 1, wherein the combination comprises seeds and the first endophyte is associated with the seeds as a coating on the surface of the seeds.

6. The synthetic combination of claim 1, wherein the combination comprises seedlings and the first endophyte is contacted with the seedlings as a spray applied to one or more leaves and/or one or more roots of the seedlings.

7. The synthetic combination of claim 1 comprising a plurality of seeds, wherein the first endophytes are present in an amount of at least about 100 CFU or spores, at least 300 CFU or spores, at least 1,000 CFU or spores, at least 3,000 CFU or spores, at least 10,000 CFU or spores, at least 30,000 CFU or spores, at least 100,000 CFU or spores, at least 300,000 CFU or spores, or at least 1,000,000 CFU spores per seed.

8. A plurality of synthetic combinations of claim 1, placed in a medium that promotes plant growth, said medium selected from the group consisting of:

soil, hydroponic apparatus, and artificial growth medium.

9. A plant grown from the synthetic combination of claim 1, said plant exhibiting an improved phenotype of agronomic interest, selected from the group consisting of:

disease resistance, drought tolerance, heat tolerance, cold tolerance, salinity tolerance, metal tolerance, herbicide tolerance, chemical tolerance, improved water use efficiency, improved nitrogen utilization, improved nitrogen fixation, pest resistance, herbivore resistance, pathogen resistance, increased yield, increased yield under water-limited conditions, health enhancement, vigor improvement, growth improvement, photosynthetic capability improvement, nutrition enhancement, altered protein content, altered oil content, increased biomass, increased shoot length, increased root length, improved root architecture, increased seed weight, altered seed carbohydrate composition, altered seed oil composition, number of pods, delayed senescence, stay-green, and altered seed protein composition.

10. An agricultural product comprising a 1000 seed weight amount of a synthetic combination of claim 1.

11. The synthetic combination of claim 1, wherein the agricultural plant is a monocot.

12. The synthetic combination of claim 11, wherein the agricultural plant is corn, wheat, barley, rice, sorghum, millet, oat, triticale, rye, bamboo, or sugarcane.

13. The synthetic combination of claim 12, wherein the agricultural plant is wheat.

14. The synthetic combination of claim 1, wherein the agricultural plant is a dicot.

15. The synthetic combination of claim 14, wherein the agricultural plant is soybean, canola, rapeseed, cotton, alfalfa, cassava, potato, tomato, pea, chick pea, lentil, flax, or pepper.

16. The synthetic combination of claim 1, wherein the first endophyte is capable of utilizing proline as a sole carbon source, the benefit is growth improvement, and the agricultural plant is wheat.

17. The synthetic combination of claim 1, wherein the first endophyte is capable of utilizing proline as a sole carbon source, the benefit is drought resistance, and the agricultural plant is soybean.

18. The synthetic combination of claim 1, further comprising a second endophyte, wherein the second endophyte is of the genus Nigrospora.

19. The synthetic combination of claim 18, where in the benefit is growth improvement, and the agricultural plant is soybean.

20. The synthetic combination of claim 18, where in the benefit is growth improvement, and the agricultural plant is wheat.

21. The synthetic combination of claim 18, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO:

3685.

22. The synthetic combination of claim 1, further comprising a second endophyte, wherein the second endophyte is of the genus Fusarium.

23. The synthetic combination of claim 22, where in the benefit is growth improvement, and the agricultural plant is soybean.

24. The synthetic combination of claim 22, where in the benefit is growth improvement, and the agricultural plant is wheat.

25. The synthetic combination of claim 22, where in the benefit is increased drought resistance, and the agricultural plant is wheat.

26. The synthetic combination of claim 22, where in the benefit is increased drought resistance, and the agricultural plant is soybean.

27. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO:

3613.

28. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO:

3692.

29. The synthetic combination of claim 22, wherein the second endophyte comprises an ITS nucleic acid sequence comprising SEQ ID NO:

3695.