Methods and devices for sequencing nucleic acids in smaller batches

• US 10,222,349 B2
• Filed: 07/21/2017
• Issued: 03/05/2019
• Est. Priority Date: 02/05/2007
• Status: Active Grant

• Current Assignee: IsoPlexis Corporation (Bruker Corporation)
• Original Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Inventors: Gordon, Steven, Hagerott, Thomas, Golaski, Edmund, Olejnik, Jerzy
• Primary Examiner(s): Thomas, David C
• Application Number:
  US15/656,307
• Publication Number:
  US 20180017525A1
• Time in Patent Office: 592 Days
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: G01N 27/44726 using specific dyes, markers or binding molecules
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

Claims

1. An automated nucleic acid sequencing system, comprising:

a) flow cells comprising nucleic acid to be sequenced, said flow cells mounted on

b) a moveable support controlled by a

c) software user interface, said support configured to move flow cells to

c) reagent delivery stations, each reagent delivery station in fluidic communication with

d) reagent reservoirs configured to supply extension reagents including fluorescent nucleotide analogues,

e) an imaging platform configured to detect said fluorescent nucleotide analogues, and

f) a transfer means configured for moving at least one of said flow cells from said moveable support to said imaging platform.

2. The system of claim 1, wherein said imaging platform is associated with a camera, said camera positioned for imaging one or more flow cells.

3. The system of claim 2, wherein said imaging platform is configured for x/y movement and said imaging platform is positioned under said camera.

4. The system of claim 1, wherein said system is contained within the housing of an instrument, said software user interface mounted on said housing, said software user interface in communication with a programmable processor.

5. The system of claim 1, wherein said moveable support is a carousel.

6. The system of claim 1, further comprising an actuator configured for raising said moveable support so as to disconnect said flow cells from said stations.