Target analyte sensors utilizing microspheres
First Claim
1. A composition comprising:
- a) a substrate with a surface comprising discrete sites wherein said substrate is not a fiber optic bundle; and
b) a population of microspheres comprising first and second subpopulations, wherein said first subpopulation comprises a first bioactive agent and a first encoding parameter capable of identifying said first bioactive agent and said second subpopulation comprises a second bioactive agent and a second encoding parameter capable of identifying said second bioactive agent, wherein said first and second bioactive agents are different, and wherein said microspheres are present in a random pattern on said substrate such that said sites contain not more than one microsphere.
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Abstract
A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.
189 Citations
15 Claims
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1. A composition comprising:
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a) a substrate with a surface comprising discrete sites wherein said substrate is not a fiber optic bundle; and b) a population of microspheres comprising first and second subpopulations, wherein said first subpopulation comprises a first bioactive agent and a first encoding parameter capable of identifying said first bioactive agent and said second subpopulation comprises a second bioactive agent and a second encoding parameter capable of identifying said second bioactive agent, wherein said first and second bioactive agents are different, and wherein said microspheres are present in a random pattern on said substrate such that said sites contain not more than one microsphere. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification