Compartmentalized integrated biochips

• US 10,261,073 B2
• Filed: 10/04/2013
• Issued: 04/16/2019
• Est. Priority Date: 10/10/2012
• Status: Active Grant

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Inventors: Bechstein, Daniel Jacob Benjamin, Wang, Shan X.
• Primary Examiner(s): Eom, Robert J
• Application Number:
  US14/046,605
• Publication Number:
  US 20140100141A1
• Time in Patent Office: 2,020 Days
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures

Abstract

An integrated microfluidic biochip is provided that includes a microfluidic device, where the microfluidic device includes hollow structures, where at least one the hollow structure includes an output at a bottom surface of the microfluidic device, and a sensor plate, where the sensor plate comprises a plurality of independent surface sensors, where the microfluidic device is sealably attachable to the sensor plate, where the hollow structure output abuts the surface sensor when the microfluidic device is attached to the sensor plate.

Claims

1. An integrated microfluidic biochip, comprising:

a. a microfluidic chip, wherein said microfluidic chip comprises a hollow structure system integrated to a fluid logic layer that is pressure sealed to a control layer, wherein said control layer comprises a valve, wherein said fluid logic layer comprises a fluid logic layer hollow structure system comprising microfluidic logic input/output hollow structures, fluid logic control fluid input hollow structures, a fluid logic routing structure, and fluid logic flow channel hollow structures, wherein said control layer comprises control layer control fluid hollow structures, control layer flow channel hollow structures, control layer control channel hollow vias, and control layer control channels, wherein each said hollow structure system is separated and compartmentalized from another said hollow structure system, wherein each said hollow structure system terminates to a bottom surface of said control layer, wherein each said hollow structure system consists of a single inlet, wherein said control layer is pressure sealed to a sensor interface layer comprising sensor interface hollow vias, sensor layer flow channels, functionalized surface/reagent interfaces; and

b. a sensor plate, wherein said sensor plate comprises a plurality of sensor arrays, wherein each said sensor array is independently addressable fluidically and electronically, wherein said plurality of sensor arrays are independently coupled to switchable channels, wherein said array of sensors comprise magnetic sensors selected from the group consisting of GMR sensors, magnetic tunnel junction sensors, and hall-effect sensors, wherein each said single inlet is configured to deliver fluid to one said array of sensors, wherein said sensor interface layer is pressure sealed to said sensors according to pressure from a clamping pad, wherein each said compartmentalized hollow structure system abuts one said sensor when said fluid logic layer is attached to said sensor plate, wherein each said independent sensor of said array of sensors comprises an independent and distinct capture molecule deposited thereon.

2. The integrated microfluidic biochip of claim 1, wherein said microfluidic biochip comprises a plurality of microfluidic layers, where said plurality of microfluidic layers are interconnected by hollow vias.

3. The integrated microfluidic biochip of claim 2, wherein said interconnected plurality of layers sealably interface a microfluidic chip, wherein said microfluidic chip is larger than a surface of said sensor plate.

4. The integrated microfluidic biochip of claim 3, wherein i) said array of hollow structure systems, ii) said independent surface sensors, iii) said microfluidic chip, i) and ii), i) and iii), ii) and iii), or i) and ii) and iii) are disposed to hold an analyte, wherein said analyte is held in an individual said hollow structure.

5. The integrated microfluidic biochip of claim 2, wherein said microfluidic layers comprises control channels and flow channels, wherein said control channels comprise i) said valve, ii) said pump or i) and ii), wherein said control channels control fluid in said flow channels in an adjacent said microfluidic layer by operating said pump and by closing and opening said valves in said flow channels in said adjacent microfluidic layer.

6. The integrated microfluidic biochip of claim 1, wherein said array of hollow structure systems are arranged to isolate individual said independent surface sensors or groups of said independent surface sensors from other said independent surface sensors on said sensor plate.

7. The integrated microfluidic biochip of claim 1, wherein said control layer comprises independent microfluidic valves, wherein said independent microfluidic valves are either disposed in an open position or closed position to dynamically program said array of hollow structure systems.

8. The integrated microfluidic biochip of claim 1, wherein said independent and distinct capture molecule comprises an antibody, an antigen, or an oligonucleotide.

9. The integrated microfluidic biochip of claim 1, wherein magnetic sensors are individually addressed electronically by rows of conductive wires and by columns of conductive wires.

10. The integrated microfluidic biochip of claim 1, wherein said magnetic sensors are individually addressed electronically by multiplexing and demultiplexing selector circuits.