De novo synthesized gene libraries
First Claim
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1. A device for synthesizing oligonucleotides, the device comprising:
- a silicon plate having formed therein;
a plurality of first channels, wherein each first channel extends vertically to a depth into the silicon plate from an opening on a top side of the silicon plate, and wherein each first channel has a width of 0.5 mm to 1.5 mm; and
for each first channel, 50 to 500 second channels in fluid communication with the first channel, wherein each second channel extends vertically to a height from an opening on a bottom side of the silicon plate to the first channel, wherein each second channel has a width of 1 um to 100 um, wherein a ratio of width to height of each second channel is from 1 to 0.1, wherein each second channel comprises a plurality of oligonucleotides bound to a first interior surface of said second channel, wherein each of the oligonucleotides comprises at least 80 nucleotides in length, and wherein the device provides for rapid exchange of chemical exposure during de novo synthesis of oligonucleotides, andwherein the first interior surface of said second channel comprises a first set of molecules, wherein each molecule of the first set of molecules is an aminosilane or N-(3-triethoxvsilvlpropvD-4-hydroxybutvramide.
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Abstract
Provided herein are devices for the manufacturing of high-quality oligonucleic acids. Longer nucleic acids, e.g., genes, can be synthesized in parallel using microfluidic assemblies described herein. Devices described herein include silicon plates having a plurality of channels in fluid communication with a plurality of microchannels. The number of microchannels and dimensions of the microchannels provide for rapid exchange of chemical exposure during de novo synthesis of oligonucleic acids.
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19 Claims
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1. A device for synthesizing oligonucleotides, the device comprising:
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a silicon plate having formed therein; a plurality of first channels, wherein each first channel extends vertically to a depth into the silicon plate from an opening on a top side of the silicon plate, and wherein each first channel has a width of 0.5 mm to 1.5 mm; and for each first channel, 50 to 500 second channels in fluid communication with the first channel, wherein each second channel extends vertically to a height from an opening on a bottom side of the silicon plate to the first channel, wherein each second channel has a width of 1 um to 100 um, wherein a ratio of width to height of each second channel is from 1 to 0.1, wherein each second channel comprises a plurality of oligonucleotides bound to a first interior surface of said second channel, wherein each of the oligonucleotides comprises at least 80 nucleotides in length, and wherein the device provides for rapid exchange of chemical exposure during de novo synthesis of oligonucleotides, and wherein the first interior surface of said second channel comprises a first set of molecules, wherein each molecule of the first set of molecules is an aminosilane or N-(3-triethoxvsilvlpropvD-4-hydroxybutvramide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification