Automatic response/light measurement device and method therefor

• US 10,273,533 B2
• Filed: 09/13/2016
• Issued: 04/30/2019
• Est. Priority Date: 02/04/2011
• Status: Active Grant

• Current Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Original Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Inventors: Tajima, Hideji
• Primary Examiner(s): Whatley, Benjamin R
• Application Number:
  US15/263,463
• Publication Number:
  US 20170058321A1
• Time in Patent Office: 959 Days
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00376 Conductive heating, e.g. heated plates
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1076 plurality or independently movable heads
• CPC Class Codes: G01N 21/13 Moving of cuvettes or solid samples to or from the investigating station handling materials for automatic analysis G01N35/00
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 2203/0226 High temperature; Heating means
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1065 Multiple transfer devices

Abstract

The invention relates to an automatic response/light measurement device and a method therefor, and the purpose is to effectively and quickly perform an optical measurement relating to a reaction with high reliability without increasing a device size. The device is configured to have: a container group in which a plurality of reaction containers are arranged; a measurement mount provided with a plurality of coupling ends that are joinable with apertures of the reaction containers, and have light guide portions that optically connect with the interior of the joined reaction containers; a mount transfer mechanism; a measuring device having a measuring end having at least one light guide portion that is optically connectable to the light guide portions of the coupling ends, that is able to receive light based on an optical state within the reaction containers; an on-mount measuring end transfer mechanism; and a measurement control portion.

Claims

1. An automatic response/light measurement device comprising:

a container group in which two or more reaction containers and a plurality of liquid housing parts are arranged, the liquid housing parts containing;

one or more reaction solutions usable for various reactions, one or more magnetic particle suspensions in which magnetic particles able to capture a target compound are suspended, one or more samples containing the target compound, and one or more solutions for separating and extracting the target compound; and

a nozzle head, comprising;

a suction-discharge mechanism able to suction and discharge gases;

two or more nozzles connected to the suction-discharge mechanism and movable relative to the container group, wherein a dispensing tip is detachably mountable to each of the nozzles so that suction and discharge of gases by the suction-discharge mechanism causes suction and discharge of liquids by the dispensing tips;

a magnetic force part including a magnet able to apply a magnetic field to the dispensing tips so that the magnetic particles are adsorbed to respective inner walls of the dispensing tips mounted on said nozzles, and to remove the magnetic field from the dispensing tips so that the magnetic particles are resuspended;

a measurement mount from which two or more coupling ends extend, each of the coupling ends having a light guide portion, wherein the measurement mount;

defines a movement path along which the coupling ends are arranged, and

is movable relative to the container group to simultaneously directly or indirectly join the coupling ends with respective apertures of the reaction containers so that the light guide portions of the coupling ends are optically connected with respective interiors of the reaction containers; and

a measuring device including one or more measuring ends each having a light guide portion that is sequentially optically connectable with the respective light guide portions of the coupling ends so that the measuring device is able to receive light via the one or more measuring ends based on optical states within the respective reaction containers;

wherein the suction-discharge mechanism and the magnetic force part, in combination, are operable to;

separate the target compound from each of the one or more samples, and

discharge the separated target compound in one or more of the reaction containers with at least one of the one or more reaction solutions;

and

wherein, upon the coupling ends being simultaneously directly or indirectly joined with the respective apertures of the reaction containers, the one or more measuring ends are movable along the movement path and relative to the measurement mount to sequentially optically connect the light guide portion of each of the one or more measuring ends with the respective light guide portions of the coupling ends so that the measuring device receives light sequentially from within each of the respective reaction containers.

2. The automatic response/light measurement device according to claim 1, wherein the measuring device includes a plurality of specific wavelength measuring devices capable of receiving light of specific wavelengths or wavelength bands;

wherein the one or more measuring ends comprise multiple measuring ends each corresponding to one of the specific wavelength measuring devices and being sequentially optically connectable with the respective interiors of the reaction containers; and

wherein the measuring ends are bundled together so that, when the coupling ends are simultaneously directly or indirectly joined with the respective apertures of the reaction containers, the bundled measuring ends are together movable along the movement path and relative to the measurement mount to sequentially optically connect the light guide portions of the bundled measuring ends with the respective light guide portions of the coupling ends so that the respective specific wavelength measuring devices each receive light sequentially from within each of the respective reaction containers.

3. The automatic response/light measurement device according to claim 1, wherein the container group includes sealing lids each having transparency and being able to seal the respective reaction containers by fitting with the apertures of the reaction containers, the sealing lids being mountable on the coupling ends, wherein the measuring device is able to receive the light based on the optical states within the respective reaction containers via the respective coupling ends and the respective sealing lids, and wherein the sealing lids are simultaneously mountable on said coupling ends via movement of the nozzles relative to the container group.

4. The automatic response/light measurement device according to claim 1, wherein the measurement mount of the nozzle head is movable in a vertical direction relative to at least one other component of the nozzle head.

5. The automatic response/light measurement device according to claim 1, further comprising a heating portion able to heat the respective coupling ends.

6. The automatic response/light measurement device according to claim 1, further comprising:

a temperature controller that includes a temperature source provided so as to contact or be adjacent to a lower side wall section of at least one of the reaction containers, the temperature source being operable to increase or decrease a temperature inside the at least one of the reaction containers; and

a heating portion provided so as to contact or be adjacent to an upper side wall section of the at least one of the reaction containers located above the lower side wall section of the at least one of the reaction containers, the heating unit including a heat source operable to heat the upper side wall section.

7. The automatic response/light measurement device according to claim 1, wherein the container group comprises two or more exclusive regions each corresponding to at least one of the respective nozzles of the nozzle head so that each of the nozzles is capable of entering one of the exclusive regions while not entering others of the exclusive regions;

wherein the respective exclusive regions each include;

at least one of the reaction containers,

one or more of the liquid housing parts containing at least one of the one or more reaction solutions used in the reactions,

at least one of the liquid housing parts containing at least one of the one or more magnetic particle suspensions in which the magnetic particles able to capture the target compound are suspended,

at least one of the liquid housing parts containing at least one of the one or more samples containing the target compound,

one or more of the liquid housing parts containing at least one of the one or more solutions for separating and extracting the target compound, and

at least one sealing lid transportable to the at least one of the reaction containers to seal the at least one of the one or more reaction solutions contained in the at least one of the reaction containers; and

wherein the mount is able to extend across all of the exclusive regions so that each of the coupling ends of the measurement mount is capable of entering one of the exclusive regions while not entering others of the exclusive regions.

8. The automatic response/light measurement device according to claim 1, wherein the suction-discharge mechanism and the magnetic force part, in combination, are operable to separate the target compound from each of the one or more samples by:

mixing, in the dispensing tips using the suction-discharge mechanism, each of the one or more samples with at least one of the one or more magnetic particle suspensions,

capturing the target compound on the magnetic particles,

adsorbing, using the magnetic force part, the magnetic particles to the respective inner walls of the dispensing tips mounted on the nozzles,

discharging a fluid portion of the at least one of the one or more magnetic particle suspensions from the dispensing tips,

suctioning at least one of the one or more solutions for separating and extracting the target compound into the dispensing tips, and

resuspending, using the magnetic force part, the magnetic particles in the at least one of the one or more solutions for separating and extracting the target compound.

9. The automatic response/light measurement device according to claim 4, further comprising a motor, a timing belt, and one or more sprockets that are together able to move the measurement mount of the nozzle head in the vertical direction relative to the at least one other component of the nozzle head.