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Electrode array device having an adsorbed porous reaction layer

  • US 10,286,377 B1
  • Filed: 05/15/2016
  • Issued: 05/14/2019
  • Est. Priority Date: 11/18/2004
  • Status: Active Grant
First Claim
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1. A process for forming an electrode array device for synthesis of an oligonucleotide having a plurality of electrodes, where the plurality of electrodes have a top electrode surface, and a porous reaction layer adsorbed to the top electrode surface for improved synthesis quality comprising:

  • (a) providing a substrate including a top electrode surface, one or more recesses in the substrate and a plurality of electrodes positioned in the one or more recesses, where each of the plurality of electrodes is electronically connected to a computer control system, where the computer control system is configured to control an addressing system for selecting and controlling one or more individual electrodes within the plurality of electrodes and a source to supply a current, where the source is electronically connected to each of the plurality of electrodes by the addressing system;

    (b) contacting a treatment solution to the top electrode surface, where the treatment solution comprises a chemical species that adsorbs onto the top electrode surface and a solvent capable of dissolving the chemical species;

    (c) washing off the treatment solution while leaving a layer of the chemical species adsorbed onto the top electrode surface to form a porous reaction layer; and

    (d) performing a plurality of electrochemical synthesis reactions to synthesize an oligonucleotide on the porous reaction layer, where the computer control system is configured such that the current generates a reactive species in the one or more individual electrodes within the plurality of electrodes, where the generation of the reactive species in the one or more individual electrodes within the plurality of electrodes forms a virtual flask adjacent the one or more individual electrodes within the plurality of electrodes, where the plurality of electrochemical synthesis reactions occur on the porous reaction layer within the virtual flask.

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