Instrument and method for sealed penetration of rigid packaging to measure internal oxygen concentration with an optical oxygen analyzer

• US 10,295,514 B1
• Filed: 10/17/2016
• Issued: 05/21/2019
• Est. Priority Date: 10/17/2016
• Status: Active Grant

• Current Assignee: MOCON, Inc. (Ametek Incorporated)
• Original Assignee: MOCON, Inc. (Ametek Incorporated)
• Inventors: Eastman, John
• Primary Examiner(s): Raevis, Robert R
• Application Number:
  US15/295,205
• Time in Patent Office: 946 Days
• Field of Search: 73 106, 73 232, 73 2321, 73 2327, 73 2331, 73 2332, 73 3101, 73 3105, 356 43- 50, 356244, 356246, 356436-442, 422 83, 250361 C, 2504831-4881, 362 44, 436160, 436127-138
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 31/225 for oxygen, e.g. including dissolved oxygen
• CPC Class Codes: G01N 33/0036 Specially adapted to detect a particular component all the other sub-groups of G01N33/0004 take precedence
• CPC Class Codes: G01N 33/0062 concerning the measuring method, e.g. intermittent, or the display, e.g. digital

Abstract

An assembly and method of using the assembly. The assembly is operable when assembled as a benchtop instrument for sealed penetration of an oxygen sensitive luminescent sensor into sensible communication with an enclosed space of a sealed rigid package to measure oxygen concentration therein, and operable when disassembled as a handheld instrument for sealed penetration of the oxygen sensitive luminescent sensor into sensible communication with an enclosed space of a flexible package to measure oxygen concentration therein. The assembly includes a mechanical punch press equipped with a longitudinally reciprocable fixture equipped with a hollow piercing member, and an oxygen analyzer releasably attachable to the fixture.

Claims

1. An assembly, comprising:

(a) a first component comprising a mechanical punch press equipped with a longitudinally reciprocable fixture having a sharp-tipped, rigid, lumen defining, hollow piercing member extending in a first longitudinal direction from the fixture, and a longitudinal bore extending completely through the fixture and into fluid communication with the lumen of the piercing member, and

(b) a second component comprising an oxygen analyzer having an oxygen sensitive luminescent sensor retained within a rigid, lumen defining, hollow, side-port needle proximate a distal end of the needle, with the fixture and the oxygen analyzer configured and arranged relative to one another for sealed, detachable, mounting of the oxygen analyzer onto the fixture with the needle extending into the bore of the fixture with the distal tip of the needle positioned within the lumen of the piercing member,

(c) wherein (—

) the assembly is operable, when the first and second components are assembled, as a benchtop instrument for sealed penetration of the oxygen sensitive luminescent sensor into sensible communication with an enclosed space of a sealed rigid package, and (—

) the oxygen analyzer component of the assembly is operable, when the first and second components are disassembled, as a handheld instrument for sealed penetration of the oxygen sensitive luminescent sensor into sensible communication with an enclosed space of a flexible package.

2. The assembly of claim 1 wherein the fixture is releasably attached to the mechanical punch press by a clamp.

3. The assembly of claim 2 wherein the mechanical punch press is a self-standing bench punch press having a base, a vertical support post upon which the clamp is reciprocably mounted, and a manually actuatable lever for effecting reciprocation of the clamp as between a rest position further from the base and a working position closer to the base.

4. The assembly of claim 3 wherein the reciprocable clamp is a clamping collar.

5. A method of serially measuring oxygen concentration inside each of a sealed rigid package and a sealed flexible package, comprising the steps of:

(a) obtaining an assembly in accordance with claim 3,

(b) piercing the rigid package with the piercing member by actuation of the mechanical punch press so as to cause the piercing member to penetrate into the sealed rigid package with the fixture sealingly engaging the sealed rigid package,

(c) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed rigid package,

(d) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed rigid package,

(e) converting the obtained value to an oxygen concentration based upon a known conversion algorithm, and

(f) visually reporting the oxygen concentration,

(g) disassembling the first and second components and thereafter,

(h) piercing the flexible package with the needle of the oxygen analyzer,

(i) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed flexible package,

(j) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed flexible package,

(k) converting the obtained value to an oxygen concentration based upon a known conversion algorithm, and

(l) visually reporting the oxygen concentration.

6. A method of serially measuring oxygen concentration inside each of a sealed rigid package and a sealed flexible package, comprising the steps of:

(a) obtaining an assembly in accordance with claim 3,

(b) adhering a septum to the exterior of the rigid package,

(c) piercing the rigid package with the piercing member by actuation of the mechanical punch press so as to cause the piercing member to penetrate through the septum and into the sealed rigid package with the fixture sealingly engaged against the septum,

(d) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed rigid package,

(e) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed rigid package,

(f) converting the obtained value to an oxygen concentration based upon a known conversion algorithm,

(g) visually reporting the oxygen concentration,

(h) disassembling the first and second components and thereafter,

(i) piercing the flexible package with the needle of the oxygen analyzer,

(j) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed flexible package,

(k) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed flexible package,

(l) converting the obtained value to an oxygen concentration based upon a known conversion algorithm, and

(m) visually reporting the oxygen concentration.

7. A method of serially measuring oxygen concentration inside each of a sealed rigid package and a sealed flexible package, comprising the steps of:

(a) obtaining an assembly in accordance with claim 3,

(b) placing the rigid package upon the base of the mechanical punch press,

(c) adhering a septum to an upward facing exterior surface of the rigid package,

(d) manually actuating the lever of the mechanical punch press to move the reciprocable clamp and thereby the fixture retained by the reciprocable clamp towards the septum until the piercing member penetrates through the septum and into the sealed rigid package with the fixture sealingly engaging the septum,

(e) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed rigid package,

(f) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed rigid package,

(g) converting the obtained value to an oxygen concentration based upon a known conversion algorithm, and

(h) visually reporting the oxygen concentration, and

(i) disassembling the first and second components and thereafter,

(j) adhering a septum to an upward facing exterior surface of the flexible package,

(k) piercing the flexible package with the needle of the oxygen analyzer through the septum,

(l) allowing the oxygen concentration in sensing communication with the oxygen sensitive luminescent sensor to equilibrate with the oxygen concentration in the sealed flexible package,

(m) activating the oxygen analyzer whereby the oxygen analyzer optically interrogates the oxygen sensitive luminescent sensor to obtain a value representative of the partial pressure of oxygen in the sealed flexible package,

(n) converting the obtained value to an oxygen concentration based upon a known conversion algorithm, and

(o) visually reporting the oxygen concentration.

8. The assembly of claim 1 wherein the piercing member longitudinally extends between 0.5 and 2 cm from the fixture.

9. The assembly of claim 1 wherein the fixture has a volume of 50 to 100 cm³ inclusive of internal void volume.

10. The assembly of claim 1 wherein the oxygen sensitive luminescent sensor includes an oxygen sensitive porphyrin dye retained within an oxygen permeable matrix, and the oxygen analyzer is a fiber optic oxygen analyzer further equipped with a source of excitation energy and a detector for sensing and measuring luminescent energy emitted by the oxygen sensitive porphyrin dye when excited.