Methods for purifying cells derived from pluripotent stem cells
First Claim
1. A method of enriching a population of primitive gut tube cells comprising:
- a) differentiating a population of human pluripotent stem cells into definitive endoderm cells with a TGF-β
receptor agonist;
b) differentiating the definitive endoderm cells into a population containing primitive gut tube cells;
c) enriching the population of primitive gut tube cells by contacting the population of cells with a reagent that is capable of binding the Lif receptor, and selecting for primitive gut tube cells that are bound to the reagent capable of binding the Lif receptor.
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Abstract
The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention provides methods of characterization of cells differentiated into cells expressing markers characteristic of the pancreatic endocrine lineage utilizing unique surface markers. The present invention also provides methods to enrich or sort cells expressing markers characteristic of the pancreatic endocrine lineage. The present invention also provides methods to deplete cells that may contaminate populations of cells expressing markers characteristic of the pancreatic endocrine lineage formed by the methods of the present invention, thereby reducing the incidence of tumor formation in vivo following transplantation.
176 Citations
15 Claims
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1. A method of enriching a population of primitive gut tube cells comprising:
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a) differentiating a population of human pluripotent stem cells into definitive endoderm cells with a TGF-β
receptor agonist;b) differentiating the definitive endoderm cells into a population containing primitive gut tube cells; c) enriching the population of primitive gut tube cells by contacting the population of cells with a reagent that is capable of binding the Lif receptor, and selecting for primitive gut tube cells that are bound to the reagent capable of binding the Lif receptor. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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- 8. A method of enriching a population of human primitive gut tube cells comprising differentiating a population of human pluripotent stem cells into a population containing human primitive gut tube cells, contacting the population of cells with a reagent that is capable of binding the Lif receptor, and selecting for primitive gut tube cells that are bound to the reagent capable of binding the Lif receptor.
Specification