Non-enzymatic glucose sensor

• US 10,330,634 B2
• Filed: 02/14/2017
• Issued: 06/25/2019
• Est. Priority Date: 09/06/2012
• Status: Active Grant

• Current Assignee: Amrita Vishwa Vidyapeetham Deemded University
• Original Assignee: Amrita Vishwa Vidyapeetham Deemded University
• Inventors: Thekkedath, Satheesh Babu G, Thiagarajan, Ramachandran, Nair, Bipin G
• Primary Examiner(s): Sellman, Cachet I
• Application Number:
  US15/431,801
• Publication Number:
  US 20170168007A1
• Time in Patent Office: 861 Days
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3275 Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
• CPC Class Codes: G01N 27/3278 involving nanosized elements, e.g. nanogaps or nanoparticles nanopores G01N33/48721; magnetic beads G01N27/745

Abstract

A non-enzymatic glucose sensor and method for fabricating the sensor are disclosed. The glucose sensor contains at least one non-enzymatic electrode configured to catalyze the electro-oxidation of glucose in preference to other bio-molecules. The surface of the electrode comprises CuO nanoparticles. The sensor shows sensitivity and selectivity exceeding enzyme based devices presently in use.

Claims

1. A method for fabricating a glucose sensor with at least one active electrode configured to catalyze the electro-oxidation of glucose in preference to other bio-molecules, the method comprising:

providing an insulating base plate/substrate;

forming one or more disposable silver-based electrodes on the substrate;

coating conducting carbon ink over the electrodes;

coating a slurry of CuO nanoparticles in NaOH over one of the electrodes, thereby forming the active electrode; and

encapsulating the active electrode in an insulating film layer in which a sensing window is provided.

2. The method of claim 1, wherein the size of the CuO nanoparticles ranges from 5 nm to 500 nm.

3. The method of claim 1, wherein the CuO nanoparticles are spherical particles prepared by annealing copper hydroxide above 400°

C.

4. The method of claim 1, wherein the CuO nanoparticles are elongated particles prepared by annealing copper hydroxide complex at 400°

C. for three hours.

5. The method of claim 4, wherein the CuO nanoparticles are nanowires prepared by annealing copper hydroxide at around 180°

C.

6. The method of claim 1, wherein the glucose sensor is configured to produce linear response currents of 0.3 to 1 mA when exposed to glucose concentrations in the 3-10 mM range.

7. The method of claim 6, wherein the glucose sensor is further configured to produce linear response currents of 0.1 to 0.3 mA when exposed to glucose concentrations in the 1-3 mM range.