Method for increasing the specific production rate of eukaryotic cells
DCFirst Claim
1. A method for increasing specific productivity (qP) of a recombinant Chinese Hamster Ovary (CHO) cell that produces an exogenous polypeptide, the method comprising culturing the CHO cell in a culture medium comprising meta-tyrosine at a concentration of from 0.2 mM to 0.7 mM, whereby the specific productivity (qP) is increased by at least 5% compared to an identical culturing process without supplementation of meta-tyrosine in the culture medium.
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Abstract
The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.
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11 Claims
- 1. A method for increasing specific productivity (qP) of a recombinant Chinese Hamster Ovary (CHO) cell that produces an exogenous polypeptide, the method comprising culturing the CHO cell in a culture medium comprising meta-tyrosine at a concentration of from 0.2 mM to 0.7 mM, whereby the specific productivity (qP) is increased by at least 5% compared to an identical culturing process without supplementation of meta-tyrosine in the culture medium.
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