Systems and methods for fluid and component handling
First Claim
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1. A method of performing at least three different assays selected from immunoassays, cytometric assays, and general chemistry assays on a biological sample, the method comprising:
- a) introducing a biological sample having a volume of no greater than 500 microliters into a benchtop point-of-care sample processing device, wherein the device comprises a housing for containing all of the following;
i) a sample handling system comprising a pipette assembly;
ii) a first detection station comprising an optical sensor;
iii) a second detection station comprising an optical sensor and a light source;
iv) a cytometry station comprising an imaging device and a stage for receiving a microscopy cuvette; and
v) a fluidically isolated sample collection unit configured to retain a biological sample;
vi) an assay station comprising at least a first, a second, a third, and a fourth independently movable and fluidically isolated assay unit;
vii) a communication unit configured to electronically connect said biological sample processing device with another device or with a network; and
viii) a controller, wherein the controller comprises a local memory and is operatively coupled to the sample handling system, the detection station, and the communication unit;
b) with the aid of the pipette assembly, transferring a portion of the biological sample to each of the first, second, third, and fourth assay units, wherein a different assay is performed in each of the first, second, third, and fourth assay units;
c) with the aid of the pipette assembly, transferring the first, second, third, and fourth assay units to the detection station or cytometry station, wherein assay units comprising immunoassays or general chemistry assays are transferred to the detection station and assay units comprising cytometric assays are transferred to the cytometry station;
d) with the aid of the detection station or cytometry station, obtaining data measurements of the assay performed in each of the first, second, third, and fourth assay units.
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Abstract
Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
601 Citations
13 Claims
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1. A method of performing at least three different assays selected from immunoassays, cytometric assays, and general chemistry assays on a biological sample, the method comprising:
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a) introducing a biological sample having a volume of no greater than 500 microliters into a benchtop point-of-care sample processing device, wherein the device comprises a housing for containing all of the following; i) a sample handling system comprising a pipette assembly; ii) a first detection station comprising an optical sensor; iii) a second detection station comprising an optical sensor and a light source; iv) a cytometry station comprising an imaging device and a stage for receiving a microscopy cuvette; and v) a fluidically isolated sample collection unit configured to retain a biological sample; vi) an assay station comprising at least a first, a second, a third, and a fourth independently movable and fluidically isolated assay unit; vii) a communication unit configured to electronically connect said biological sample processing device with another device or with a network; and viii) a controller, wherein the controller comprises a local memory and is operatively coupled to the sample handling system, the detection station, and the communication unit; b) with the aid of the pipette assembly, transferring a portion of the biological sample to each of the first, second, third, and fourth assay units, wherein a different assay is performed in each of the first, second, third, and fourth assay units; c) with the aid of the pipette assembly, transferring the first, second, third, and fourth assay units to the detection station or cytometry station, wherein assay units comprising immunoassays or general chemistry assays are transferred to the detection station and assay units comprising cytometric assays are transferred to the cytometry station; d) with the aid of the detection station or cytometry station, obtaining data measurements of the assay performed in each of the first, second, third, and fourth assay units. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of processing a biological sample, comprising:
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a) introducing a sample having a volume of 500 microliters or less into a benchtop, point-of-care biological sample processing device comprising a housing for containing all of the following; i) a sample handling system comprising a pipette assembly; ii) at least a first and a second fluidically isolated vessel; iii) a diluent; and iv) a centrifuge, wherein the sample comprises bodily fluid at a first concentration; v) a first detection station comprising an optical sensor; vi) a second detection station comprising an optical sensor and a light source; vii) a cytometry station comprising an imaging device and a stage for receiving a microscopy cuvette; viii) a fluidically isolated sample collection unit configured to retain a biological sample; ix) an assay station comprising at least a first, a second, a third, and a fourth independently movable and fluidically isolated assay unit; x) a communication unit configured to electronically connect said biological sample processing device with another device or with a network; and xi) a controller, wherein the controller comprises a local memory and is operatively coupled to the sample handling system, one of the first or second detection station, and the communication unit; b) with the aid of the sample handling system, introducing at least a portion of the sample into the centrifuge; c) centrifuging the sample, to generate a centrifuged sample; d) with the aid of the pipette assembly, removing at least a portion of the centrifuged sample from the centrifuge; and e) with the aid of the pipette assembly, mixing at least a portion of the centrifuged sample with the diluent to generate a diluted sample, wherein the diluted sample comprises bodily fluid at a second concentration, and the second concentration of bodily fluid is one-half or less of the first concentration of bodily fluid; and
,f) with the aid of the pipette assembly, transferring at least a portion of the diluted sample to at least one of the first and the second fluidically isolated vessels. - View Dependent Claims (9, 10, 11, 12, 13)
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Specification