Digital microscope apparatus, method of searching for in-focus position thereof, and program

• US 10,371,931 B2
• Filed: 04/12/2016
• Issued: 08/06/2019
• Est. Priority Date: 03/14/2013
• Status: Expired due to Fees

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Yamamoto, Takashi, Oshima, Takuya, Narusawa, Ryu, Yamakoshi, Takamichi, Tanabe, Norihiro
• Primary Examiner(s): Brown, Jr., Howard D
• Application Number:
  US15/097,058
• Publication Number:
  US 20160223804A1
• Time in Patent Office: 1,211 Days
• CPC Class Codes: G02B 21/245 using auxiliary sources, detectors
• CPC Class Codes: G02B 21/247 Differential detectors
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence
• CPC Class Codes: G02B 7/34 using different areas in a pupil plane

Abstract

A digital microscope apparatus includes a first imaging unit including a first imaging device and a first optical system including an objective lens configured to enlarge first and second images of a preparation that holds a sample, the first image being formed on the first device through the first system, a second imaging unit including a second optical system that is branched from the first system and has a depth of field larger than the first system and a second imaging device on which the second image is formed through the second system, and a controller configured to calculate a provisional in-focus position of the lens based on the second image, determine an area of the first device, from which the first image is read, and search for, based on the read image, an in-focus position of the lens in a predetermined range based on the provisional in-focus position.

Claims

1. An imaging control apparatus, comprising:

a controller configured to;

calculate a provisional in-focus position based on an image by phase difference auto focus;

based on a result of a detection of the image and using the provisional in-focus position as a reference, search for an in-focus position in a predetermined range in an optical axis direction;

based on the result of the detection of the image, search for the in-focus position based on an observed surface determined using the provisional in-focus position as a reference; and

output the in-focus position.

2. The imaging control apparatus according to claim 1, wherein the controller is further configured to:

acquire signals of a set of phase difference images that are formed on an imaging surface of a first imaging device.

3. The imaging control apparatus according to claim 2, wherein the controller is further configured to:

calculate an amount of defocus and an orientation of the defocus based on a distance between the set of phase difference images.

4. The imaging control apparatus according to claim 3, wherein the provisional in-focus position is calculated based on the image, the amount of defocus, and the orientation of the defocus.

5. The imaging control apparatus according to claim 4, wherein the search is done within a reading area of the image, and the reading area is restricted to a detected area that contains a sample based on a detection of the sample in the set of phase difference images.

6. The imaging control apparatus according to claim 1, wherein the phase difference auto focus uses light split into two light beams.

7. The imaging control apparatus according to claim 6, further comprising a splitter lens, wherein the two light beams are from the splitter lens.

8. A method of searching for an in-focus position of an imaging control apparatus, comprising:

calculating a provisional in-focus position based on an image by phase difference auto focus;

based on a result of a detection of the image and using the provisional in-focus position as a reference, searching for the in-focus position in a predetermined range in an optical axis direction;

based on the result of the detection of the image, searching for the in-focus position on an observed surface determined using the provisional in-focus position as a reference; and

outputting the in-focus position.

9. The method according to claim 8, further comprising:

acquiring signals of a set of phase difference images that are formed on an imaging surface of a first imaging device.

10. The method according to claim 9, further comprising:

calculating an amount of defocus and an orientation of the defocus based on a distance between the set of phase difference images.

11. The method according to claim 10, wherein the provisional in-focus position is calculated based on the image, the amount of defocus, and the orientation of the defocus.

12. The method according to claim 11, wherein the search is done within a reading area of the image, and the reading area is restricted to a detected area that contains a sample based on a detection of the sample in the set of phase difference images.

13. The method according to claim 8, wherein the phase difference auto focus uses light split into two light beams.

14. The method according to claim 13, further comprising a splitter lens, wherein the two light beams are from the splitter lens.

15. A system, comprising:

a microprocessor coupled to an imaging control device, the microprocessor programmed to;

calculate a provisional in-focus position based on an image by phase difference auto focus;

based on a result of a detection of the image and using the provisional in-focus position as a reference, search for an in-focus position in a predetermined range in an optical axis direction;

based on the result of the detection of the image, search for the in-focus position on an observed surface determined using the provisional in-focus position as a reference; and

output the in-focus position.

16. The system according to claim 15, wherein the microprocessor is further programmed to:

acquire signals of a set of phase difference images that are formed on an imaging surface of a first imaging device.

17. The system according to claim 16, wherein the microprocessor is further programmed to:

calculate an amount of defocus and an orientation of the defocus based on a distance between the set of phase difference images.

18. The system according to claim 17, wherein the provisional in-focus position is calculated based on the image, the amount of defocus, and the orientation of the defocus.

19. The system according to claim 18, wherein the search is done within a reading area of the image, and the reading area is restricted to a detected area that contains a sample based on a detection of the sample in the set of phase difference images.

20. The system according to claim 15, wherein the phase difference auto focus uses light split into two light beams.

21. The system according to claim 20, further comprising a splitter lens, wherein the two light beams are from the splitter lens.