System and method for laser imaging and ablation of cancer cells using fluorescence

• US 10,376,148 B2
• Filed: 12/05/2013
• Issued: 08/13/2019
• Est. Priority Date: 12/05/2012
• Status: Active Grant

• Current Assignee: AccuVein LLC
• Original Assignee: AccuVein LLC
• Inventors: Wood, Fred, Yavid, Dmitry, Zott, Joe, Goldman, Ron
• Primary Examiner(s): Layno, Carl H
• Assistant Examiner(s): Xie, Dacheng
• Application Number:
  US14/097,337
• Publication Number:
  US 20140187879A1
• Time in Patent Office: 2,077 Days
• Field of Search: 606 2- 19, 607 86- 94
• CPC Class Codes: A61B 18/20 using laser
• CPC Class Codes: A61B 2018/00577 Ablation
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0077 Devices for viewing the surface of the body, e.g. camera, magnifying lens
• CPC Class Codes: A61B 5/742 using visual displays A61B5/339 takes precedence

Abstract

A fluorescence imaging device detects fluorescence in parts of the visible and invisible spectrum, and projects the fluorescence image directly on the human body, as well as on a monitor, with improved sensitivity, video frame rate and depth of focus, and enhanced capabilities of detecting distribution and properties of multiple fluorophores. Direct projection of three-dimensional visible representations of florescence on three-dimensional body areas advantageously permits view of it during surgical procedures, including during cancer removal, reconstructive surgery and wound care, etc. A NIR laser and a human visible laser (HVL) are aligned coaxially and scanned over the operating field of view. When the NIR laser passes over the area where the florescent dye is present, it energizes the dye which emits at a shifted NIR frequency detected by a photo diode. The HVL is turned on when emission is detected, providing visual indication of those positions.

Claims

1. A method for three-dimensional imaging of cancer cells of a target surgical area, and for projecting the imaged cancer cells directly onto the target surgical area, said method comprising:

introducing, fluorophores having affinity for targeted cancer cells into biologic tissues of the target surgical area;

emitting a beam of light at a first infrared wavelength from a first laser, a beam of light at a red wavelength from a second laser, a beam of light at a blue wavelength from a third laser, and a beam of light at a green wavelength from a fourth laser, within each of a plurality of alternate imaging frames;

co-axially aligning the beams of infrared, red, blue, and green light using a means for aligning;

scanning the co-axially aligned beam of light, using a scanner, in a pattern, and across the target surgical area, exciting the fluorophores and causing emitting of fluorescent excitation light at a second infrared wavelength during the alternate imaging frames, and causing illumination of the target surgical area with white light during the alternate imaging frames;

converting each image of the fluorescent excitation light of the fluorophores for each of the alternate imaging frames into an analog signal by a light detector;

converting the analog signal of each image into a digital image by an image processor, and successively storing each in a memory;

successively outputting each digital image of the stored image frames to one of said red, green, or blue lasers as an analog signal by the processing unit, and projecting by said one of said red, green, or blue lasers of each image onto the target surgical area during respective display frames succeeding the alternate imaging frames, using said analog signal.

2. The method according to claim 1 further comprising:

selectively emitting a beam of ablation light, by a fifth laser, at a selective wavelength and ablating of the targeted cancer cells;

aligning the beam of ablation light with the co-axially aligned beam of light; and

controlling said selectively emitted ablation light by the fifth laser by the image processor for only occurring when directed at the targeted cancer cells.

3. The method according to claim 1 further comprising:

transmitting, by the image processor, each stored digital image of the alternate imaging frames to a monitor for successively displaying of each image thereon.

4. The method according to claim 1 further comprising:

capturing a combined image of the target surgical area and the projection of each image on the target surgical area using a camera, and displaying said captured image on a monitor.

5. The method according to claim 4 further comprising synchronizing a frame rate of the camera with a frame rate of the scanner.

6. The method according to claim 1 wherein the first infrared wavelength of light is at a wavelength of 780 nm.

7. The method according to claim 6 wherein the red wavelength of light is at a wavelength of 640 nm.

8. The method according to claim 7 wherein said second infrared wavelength of light is approximately at a wavelength of 820 nm.

9. The method according to claim 8 wherein the blue wavelength of light is at a wavelength of 440 nm.

10. The method according to claim 9 wherein the green wavelength of light is in the range of wavelengths between 510 nm and 540 nm.