Coated surgical mesh, and corresponding systems and methods

• US 10,405,961 B2
• Filed: 09/02/2016
• Issued: 09/10/2019
• Est. Priority Date: 03/14/2013
• Status: Active Grant

• Current Assignee: Cell and Molecular Tissue Engineering, LLC
• Original Assignee: Cell and Molecular Tissue Engineering, LLC
• Inventors: Klueh, Ulrike W., Kreutzer, Donald L.
• Primary Examiner(s): Humphrey, Louise W
• Assistant Examiner(s): Patury, Srikanth
• Application Number:
  US15/255,484
• Publication Number:
  US 20170143876A1
• Time in Patent Office: 1,103 Days
• Field of Search: None
• CPC Class Codes: A61B 2562/18 Shielding or protection of sensors from environmental influences, e.g. protection from mechanical damage
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/686 Permanently implanted devices, e.g. pacemakers, other stimulators, biochips A61B5/6861 takes precedence
• CPC Class Codes: A61F 2/0063 Implantable repair or support meshes, e.g. hernia meshes
• CPC Class Codes: A61F 2240/001 Designing or manufacturing processes
• CPC Class Codes: A61F 2250/0067 Means for introducing or releasing pharmaceutical products into the body
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/64 Animal cells
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: A61M 2005/1581 Right-angle needle-type devices
• CPC Class Codes: A61M 2205/0205 Materials having antiseptic or antimicrobial properties, e.g. silver compounds, rubber with sterilising agent
• CPC Class Codes: A61M 5/158 Needles for infusions; Accessories therefor, e.g. for inserting infusion needles, or for holding them on the body
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Disclosed herein are medical products, including a surgical mesh, with a layer of dehydrated modified basement membrane preparation formed thereon. The basement membrane has been modified before dehydration of remove low molecular weight components. In some embodiments, the basement membrane is crosslinked. Methods of making and using the products also are disclosed.

Claims

1. A surgical mesh coated with a crosslinked basement membrane preparation comprising Type IV collagen and laminin, wherein the crosslinked basement membrane preparation contains no more than 0.024 mg/ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less.

2. The surgical mesh of claim 1, wherein the crosslinked basement membrane preparation is dehydrated after being in the form of a gel at room temperature.

3. The surgical mesh of claim 1, wherein the crosslinked basement membrane preparation is dehydrated at a temperature in the range of 1-4°

C. after being placed on the mesh when the basement membrane is in the form of a liquid.

4. The surgical mesh of claim 1, wherein the crosslinked basement membrane preparation is dehydrated at a temperature in the range of 10-30°

C.

5. The surgical mesh of claim 1, wherein the crosslinked basement membrane preparation is applied in multiple layers, and the basement membrane preparation is dehydrated after application of each layer.

6. The surgical mesh of claim 1, wherein the mesh also comprises layers of other extracellular matrices formed thereon.

7. The surgical mesh of claim 1, wherein the crosslinked basement membrane preparation contains at least one of cells and factors, the factors being at least one member selected from the group consisting of angiogenic factors, drugs, antimicrobials, cytokines, growth factors and anti-inflammatory factors.

8. A kit comprising the surgical mesh of claim 1 enclosed in sterile packaging.

9. The surgical mesh of claim 5, wherein the crosslinked basement membrane preparation contains at least one of cells and factors, the factors being at least one member selected from the group consisting of angiogenic factors, drugs, antimicrobials, cytokines, growth factors and anti-inflammatory factors.

10. The surgical mesh of claim 1, wherein the basement membrane preparation contains a factor, including at least one of a vascular endothelial growth factor, a hepatocyte growth factor and a fibroblast growth factor.

16. The surgical mesh of claim 10, wherein the basement membrane is crosslinked using a di-aldehyde.

17. The surgical mesh of claim 1, wherein the basement membrane in the preparation comprises cell culture derived basement membrane.

11. A surgical mesh coated with a basement membrane preparation comprising Type IV collagen and laminin, said basement membrane preparation is crosslinked with a dialdehyde, wherein said crosslinked basement membrane preparation contains no more than 0.024 mg/ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less.

12. The surgical mesh of claim 11, wherein the basement membrane preparation is applied in multiple layers, and the basement membrane preparation is dehydrated after application of each layer.

13. The surgical mesh of claim 11, wherein the surgical mesh also comprises layers of other extracellular matrices formed thereon.

14. The surgical mesh of claim 11, wherein the crosslinked basement membrane preparation contains at least one of cells and factors, the factors being at least one member selected from the group consisting of angiogenic factors, drugs, antimicrobials, cytokines, growth factors and anti-inflammatory factors.

15. The surgical mesh of claim 14, wherein the cells and/or factors are bonded to the basement membrane preparation.

18. The surgical mesh of claim 11, wherein the basement membrane in the preparation comprises cell culture derived basement membrane.