System and method for microfluidic cell culture

  • US 10,421,936 B2
  • Filed: 12/13/2017
  • Issued: 09/24/2019
  • Est. Priority Date: 07/07/2010
  • Status: Active Grant
First Claim
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1. A method for selecting a cell, or a clone thereof, from a population of cells, comprising:

  • introducing the population of cells into 1,600 to 20,000 microfluidic chambers of a microfluidic device via a single introduction port, wherein each microfluidic chamber comprises an inlet, the single introduction port is in fluid communication with a flow channel that is in fluid communication with the inlets of the microfluidic chambers, and wherein individual cells of the population are retained in unique microfluidic chambers of the microfluidic device, and the cells are transported via the introduction port and flow channel into the unique microfluidic chambers;

    providing a cell culture medium to the plurality of microfluidic chambers via the flow channel and the inlets of the chambers;

    exchanging the cell culture medium in the individual microfluidic chambers via the flow channel and the inlets of the chambers, to create a plurality of individual clonal cell populations, wherein the individual clonal cell populations are retained in the same microfluidic chamber as their respective parental cells;

    measuring a cell product secreted by the individual clonal cell populations, or subset thereof, within one or more of the microfluidic chambers; and

    selecting one or more individual clonal cell populations from the plurality to provide one or more selected individual clonal cell populations.

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