Pre-and-intra-operative localization of penile sentinel nodes

• US 10,434,190 B2
• Filed: 05/31/2017
• Issued: 10/08/2019
• Est. Priority Date: 09/07/2006
• Status: Active Grant

• Current Assignee: Stryker Corporation
• Original Assignee: Novadaq Technologies ULC (Stryker Corporation)
• Inventors: Golijanin, Dragan, Brzozowski, Lukasz, Wood, Ronald
• Primary Examiner(s): Jones, D. L.
• Application Number:
  US15/610,102
• Publication Number:
  US 20180104362A1
• Time in Patent Office: 860 Days
• Field of Search: 424 111, 424 165, 424 91, 424 92, 424 96
• CPC Class Codes: A61K 49/0034 Indocyanine green, i.e. ICG, cardiogreen

Abstract

The invention provides methods for pre- and intra-operatively localizing penile sentinel lymph nodes by injection of fluorescent dyes into penile tumors.

Claims

1. A method of localizing before or during a surgical operation a lymph node draining a penile tumor having edges and a base, in a subject, comprising:

a) 1 hour to 6 hours prior to said surgical operation, injecting the edges or the base of said tumor of said subject with 30 to 300 μ

L of a dye which fluoresces at an emission wavelength when said dye is contacted with an excitation wavelength;

b) exposing said lymph node before or during said operation to illumination using a laparoscopic instrument, the illumination comprises said excitation wavelength and causes said fluorescent dye to fluoresce; and

,

c) detecting the fluorescence of said dye at said lymph node.

2. The method of claim 1, wherein said lymph node is a sentinel lymph node.

3. The method of claim 1, wherein said dye is injected into the edges of said tumor.

4. The method of claim 1, wherein said dye is injected into the base of said tumor.

5. The method of claim 1, wherein said lymph node is visualized on an image display.

6. The method of claim 1 wherein said dye is a dye which fluoresces when exposed to near infrared light.

7. The method of claim 1, wherein said dye is a tricarbocyanine dye.

8. The method of claim 7, wherein the tricarbocyanine dye is indocyanine green.

9. The method of claim 1, wherein the subject is a human.

10. The method of claim 1, wherein said dye is injected between 1 hour and 3 hours before said surgical operation.

11. The method of claim 1, wherein said dye is injected between 1 hour and 2 hours before said surgical operation.

12. The method of claim 1, wherein said steps (b) and (c) are performed during said operation.

13. The method of claim 1, wherein said operation is a laparoscopic operation.

14. The method of claim 1, wherein step (a) requires two to five injections.