Method for transferring a target between locations

• US 10,441,950 B2
• Filed: 07/17/2018
• Issued: 10/15/2019
• Est. Priority Date: 01/14/2014
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Beebe, David J., Casavant, Ben P., Guckenberger, David J., Berry, Scott M.
• Primary Examiner(s): Conley, Sean E
• Assistant Examiner(s): Brazin, Jacqueline
• Application Number:
  US16/037,153
• Publication Number:
  US 20180318827A1
• Time in Patent Office: 455 Days
• Field of Search: 422538
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0657 Pipetting powder
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2400/021 non contact spotting by inertia, i.e. abrupt deceleration of the nozzle or pin
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors

Abstract

A device and method are proved for transferring a target from a first location to a second location. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes transfer surface for receiving the target bound solid phase substrate thereon for transfer. The transfer surface movable between a first position wherein the transfer surface is aligned with the first location and spaced therefrom by a distance and a second position wherein the transfer surface is aligned with the second location. An alignment structure aligns the transfer surface with respect to the second location, with the transfer surface in the second position. A force is movable between an attraction position wherein the target bound solid phase substrate are drawn toward the transfer surface and a discharge position wherein the target bound solid phase substrate are free of the force.

Claims

1. A method for transferring a target from a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form a target bound solid phase substrate, comprising:

forming a non-concave meniscus with the first fluid;

providing a transfer device with a lower transfer surface and an upper surface;

positioning a transfer region of the lower transfer surface of the transfer device in axial alignment with and in spaced relation to the non-concave meniscus so as to provide a non-liquid air gap therebetween;

generating a force adjacent to the upper surface of the transfer device that is sufficient to attract the target bound solid phase substrate toward the transfer region;

drawing the target bound solid phase substrate from the first fluid, through the non-liquid air gap, and to the transfer region of the transfer device with the force;

maintaining the target bound solid phase substrate adjacent to the transfer region of the transfer device with the force;

aligning the transfer region with the second location, spaced from the first location;

reducing the force on the target bound solid phase substrate such that the force is insufficient to maintain the target bound solid phase substrate adjacent to the transfer region of the transfer device so as to release the target bound solid phase substrate from the transfer region of the transfer device and allow the target bound solid phase substrate to pass to the second location.

2. The method of claim 1 wherein the step of forming the non-concave meniscus with the first fluid includes the additional steps of:

depositing the first fluid in a sample container; and

inserting an insert into the sample container, the insert causing the first fluid in the sample container to have the non-concave meniscus.

3. The method of claim 1 wherein the step of forming the non-concave meniscus with the first fluid includes the additional step of depositing the first fluid in a sample container having a hydrophobic inner surface, the hydrophobic inner surface causing the first fluid in the sample container to have the non-concave meniscus.

4. The method of claim 1 comprising the additional steps of:

positioning an intermediate fluid between the first fluid and the transfer region; and

passing the target bound solid phase substrate through the intermediate fluid as the target bound solid phase substrate are drawn from the first fluid to the transfer region.