Method for processing polynucleotide-containing samples

  • US 10,443,088 B1
  • Filed: 07/22/2019
  • Issued: 10/15/2019
  • Est. Priority Date: 05/03/2004
  • Status: Active Grant
First Claim
Patent Images

1. A method for processing a polynucleotide-containing sample, the method comprising:

  • retaining polynucleotides from a sample on a plurality of binding particles in a process chamber under a first set of conditions, wherein the retaining step comprises binding the polynucleotides to the surfaces of the plurality of binding particles comprising a poly-cationic substance, wherein the sample has a volume from 0.5 microliters to 3 milliliters;

    wherein the first set of conditions includes a first pH of about 8.5 or less and a first temperature, wherein the first temperature is between about 30°

    C. and about 50°

    C.;

    releasing the polynucleotide from the plurality of binding particles under a second set of conditions; and

    wherein the second set of conditions includes increasing the pH to a second pH by addition of a hydroxide solution and increasing the temperature to a second temperature, wherein the second temperature is between about 80°

    C. and about 100°

    C.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×