Method of separating ions

• US 10,446,385 B2
• Filed: 05/16/2017
• Issued: 10/15/2019
• Est. Priority Date: 05/17/2016
• Status: Active Grant

• Current Assignee: Micromass UK Ltd (Waters Corporation)
• Original Assignee: Micromass UK Ltd (Waters Corporation)
• Inventors: Green, Martin Raymond, Giles, Kevin, Langridge, David John
• Primary Examiner(s): McCormack, Jason L
• Application Number:
  US15/596,451
• Publication Number:
  US 20170338093A1
• Time in Patent Office: 882 Days
• Field of Search: 250281, 250282, 250283, 250290, 250291, 250292, 250293
• CPC Class Codes: G01N 2030/025 Gas chromatography
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/582 micellar electrokinetic capillary chromatography [MECC]
• CPC Class Codes: G01N 27/622 Ion mobility spectrometry
• CPC Class Codes: H01J 49/40 Time-of-flight spectrometers H01J49/36 takes precedence

Abstract

The present disclosure relates generally to a method of separating ions according to their ion mobility, comprising (i) accumulating a first population of ions in a first region of an ion mobility separator, (ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator, and (iii) accumulating a second population of ions in said first region of said ion mobility separator while said first population of ions are being separated according to their ion mobility in said ion mobility separator.

Claims

1. A method of separating ions according to their ion mobility, comprising:

(i) accumulating a first population of ions in a first region of an ion mobility separator;

(ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator and in a second region of said ion mobility separator; and

(iii) accumulating a second population of ions in said first region of said ion mobility separator after said first population of ions have exited the first region, and whilst said first population of ions are being separated according to their ion mobility in said second region of said ion mobility separator,

wherein step (ii) comprises accumulating said second population of ions in an ion trap upstream of said first region, whilst said first population of ions are being separated according to their ion mobility in said first region of said ion mobility separator, wherein said ion trap is external to said ion mobility separator,

wherein said first region of said ion mobility separator switches between a trapping mode in steps (i) and (iii), in which ions are substantially trapped within said first region and not separated according to their ion mobility, and an ion mobility separation mode in step (ii), in which ions are separated according to their ion mobility within said first region.

2. A method as claimed in claim 1, wherein step (iii) comprises transferring said second population of ions from said upstream ion trap and into said first region, and continuing to pass ions through said upstream ion trap and into said first region so as to accumulate said second population of ions in said first region.

3. A method as claimed in claim 1, wherein said ion mobility separator comprises a second region downstream of said first region, and step (ii) comprises separating said first population of ions according to their ion mobility in said first and second regions of said ion mobility separator.

4. A method as claimed in claim 3, wherein step (iii) comprises accumulating said second population of ions in said first region of said ion mobility separator whilst said first population of ions are being separated according to their ion mobility in said second region of said ion mobility separator.

5. A method as claimed in claim 1, wherein step (iii) comprises accumulating said second population of ions in said first region of said ion mobility separator after said first population of ions have exited said first region of said ion mobility separator.

6. A method as claimed in claim 1, further comprising:

(iv) separating said second population of ions according to their ion mobility in said ion mobility separator; and

(v) accumulating a third population of ions in said first region of said ion mobility separator whilst said second population of ions are being separated according to their ion mobility.

7. A method as claimed in claim 6, further comprising:

repeating steps (ii) to (v) for said third and further populations of ions, such that subsequent populations of ions are accumulated in said first region of said ion mobility separator whilst preceding populations of ions are being separated according to their ion mobility.

8. A method as claimed in claim 1, further comprising directing a beam of ions continuously into the upstream ion trap.

9. A method as claimed in claim 1, wherein said ion mobility separator comprises an RF-confined ion mobility separator.

10. A method as claimed in claim 9, wherein said ion mobility separator comprises a plurality of electrodes stacked adjacent to one another, wherein alternate phases of an RF voltage are applied to adjacent electrodes.

11. A method as claimed in claim 10, wherein each of said plurality of electrodes comprise apertures through which ions travel in use.

12. A method as claimed in claim 10, wherein said plurality of electrodes comprises a plurality of pairs of electrodes stacked adjacent to one another, wherein alternate phases of an RF voltage are applied to adjacent pairs of electrodes.

13. A method as claimed in claim 12, wherein each of said pair of electrodes comprises a first plate electrode opposite a second plate electrode, wherein in use ions travel through the gap between said first and second plate electrodes.

14. A method as claimed in claim 1, wherein said first region ion trap comprise RF-confined regions.

15. A method as claimed in claim 1, wherein said ion trap of said ion mobility separator is arranged and adapted to trap or accumulate a population of ions and then transfer the population of ions from the ion trap into the first region.

16. A method as claimed in claim 1, wherein step (i) comprises accumulating said first population of ions in said first region such that within the first region the first population of ions has a relatively small spatial spread in the direction of ion mobility separation, and a relatively large spatial spread in the direction orthogonal to ion mobility separation.

17. An ion mobility spectrometer or separation device comprising:

an ion mobility separator comprising a first region arranged and adapted to accumulate consecutive populations of ions; and

a control system arranged and adapted;

(i) to accumulate a first population of ions in said first region of said ion mobility separator;

(ii) to separate said first population of ions according to their ion mobility in said first region of said ion mobility separator and in a second region of said ion mobility separator; and

(iii) to accumulate a second population of ions in said first region of said ion mobility separator after said first population of ions have exited the first region, and whilst said first population of ions are being separated according to their ion mobility in said second region of said ion mobility separator,

wherein step (ii) comprises accumulating said second population of ions in an ion trap upstream of said first region, whilst said first population of ions are being separated according to their ion mobility in said first region of said ion mobility separator, wherein said ion trap is external to said ion mobility separator,

wherein said first region of said ion mobility separator switches between a trapping mode in steps (i) and (iii), in which ions are substantially trapped within said first region and not separated according to their ion mobility, and an ion mobility separation mode in step (ii), in which ions are separated according to their ion mobility within said first region.

18. An ion mobility spectrometer or separation device as claimed in claim 17, further comprising an ion trap upstream of said first region, wherein said control system is arranged and adapted:

to accumulate said second population of ions in said ion trap whilst said first population of ions are being separated according to their ion mobility in said first region of said ion mobility separator.

19. A method of separating ions in a device, wherein the device comprises a first region, an ion trap upstream of the first region and a second region downstream of the first region, wherein the first and second regions form part of an ion mobility separator and said ion trap is external to said ion mobility separator, wherein the method comprises the steps of:

(i) driving a first population of ions through the first and second regions so as to separate a first population of ions, whilst accumulating a second population of ions in the ion trap;

(ii) after the first population of ions has exited the first region, but prior to the first population of ions exiting the second region, transferring the second population of ions into the first region and trapping the second population of ions within the first region; and

then

(iii) driving the second population of ions through the first and second regions so as to separate the second population of ions, whilst accumulating a third population of ions in the ion trap;

(iv) repeating steps (ii) and (iii) for the third and further populations of ions so as to successively separate the third and further populations of ions,

wherein said first region switches between a trapping mode in step (ii), in which ions are substantially trapped within said first region and not separated according to their ion mobility, and an ion mobility separation mode in steps (i) and (iii), in which ions are separated according to their ion mobility within said first region.

20. An apparatus comprising a device for separating ions and a control system, wherein the device comprises a first region, an ion trap upstream of the first region and a second region downstream of the first region,

wherein the first and second regions form part of an ion mobility separator and said ion trap is external to said ion mobility separator, wherein the control system is arranged and adapted:

(i) to drive a first population of ions through the first and second regions so as to separate the first population of ions, for example according to their ion mobility, whilst accumulating a second population of ions in the ion trap;

(ii) after the first population of ions has exited the first region, but prior to the first population of ions exiting the second region, to transfer the second population of ions into the first region and trap the second population of ions within the first region; and

then

(iii) to drive the second population of ions through the first and second regions so as to separate the second population of ions, for example according to their ion mobility, whilst accumulating a third population of ions in the ion trap; and

(iv) to repeat steps (ii) and (iii) for the third and further populations of ions so as to successively separate the third and further populations of ions, for example according to their ion mobility,

wherein said first region of said ion mobility separator switches between a trapping mode in step (ii), in which ions are substantially trapped within said first region and not separated according to their ion mobility, and an ion mobility separation mode in steps (i) and (iii), in which ions are separated according to their ion mobility within said first region.