Integrated multiplex target analysis

• US 10,495,656 B2
• Filed: 07/23/2015
• Issued: 12/03/2019
• Est. Priority Date: 10/24/2012
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Genmark Diagnostics Incorporated (Roche Holding AG)
• Inventors: Kayyem, Jon Faiz, Srinivasan, Jayashankar, Ford, Sean
• Primary Examiner(s): Crow, Robert T.
• Application Number:
  US14/807,000
• Publication Number:
  US 20150323555A1
• Time in Patent Office: 1,594 Days
• Field of Search: None
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: G01N 2035/00019 cassette structures
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0091 GUI [graphical user interfaces]
• CPC Class Codes: G01N 35/00722 Communications; Identification
• CPC Class Codes: G01N 35/00732 : Identification of carriers, materials or components in automatic analysers

Abstract

This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.

Claims

1. An apparatus for detection of target analytes comprising:

(a) a biochip cartridge comprising;

(i) a deformable vessel, the deformable vessel comprising a mixing paddle; and

(ii) a reaction module; and

(b) an instrument bank comprising a biochip cartridge bay, the biochip cartridge bay holding the biochip cartridge and comprising;

(i) a processor;

(ii) a deformable vessel actuator positioned adjacent the deformable vessel of the biochip cartridge held in the biochip cartridge bay; and

(iii) a mixing motor, wherein the mixing motor in the biochip cartridge bay is coupled with the mixing paddle in the deformable vessel of the biochip cartridge held in the biochip cartridge bay.

2. The apparatus according to claim 1, wherein the deformable vessel actuator comprises an articulated deformable vessel actuator platen assembly and a sliding actuator plate.

3. The apparatus according to claim 2, wherein the sliding actuator plate comprises a cam follower which is associated with the deformable vessel.

4. The apparatus of claim 1, wherein the deformable vessel is configured to hold a volume up to 3000 μ

l and the reaction module is configured to hold a volume of less than 10 μ

l.

5. The apparatus of claim 1, wherein the biochip cartridge bay further comprises pogo pins, wherein the biochip cartridge held in the biochip cartridge bay further comprises grid electrodes, and wherein the pogo pins are connected to the grid electrodes.