Genetic engineering of non-human animals for the production of chimeric antibodies
First Claim
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1. A polynucleotide comprising a transgene encoding a polypeptide comprising an immunoglobulin heavy chain variable region, wherein the transgene comprises (1) a plurality of immunoglobulin heavy chain variable (V) exons encoding human immunoglobulin heavy chain V polypeptides;
- (2) non-coding sequences between the V exons;
(3) a plurality of immunoglobulin heavy chain joining (J) coding sequences encoding human immunoglobulin heavy chain joining J polypeptides; and
(4) non-coding sequences between the J coding sequences;
wherein the non-coding sequences between the V exons and the non-coding sequences between the J coding sequences are derived from mouse, rat, or a combination thereof immunoglobulin heavy chain non-coding sequences.
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Abstract
The invention provides non-human cells and mammals having a genome encoding chimeric antibodies and methods of producing transgenic cells and mammals. Certain aspects of the invention include chimeric antibodies, humanized antibodies, pharmaceutical compositions and kits. Certain aspects of the invention also relate to diagnostic and treatment methods using the antibodies of the invention.
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18 Claims
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1. A polynucleotide comprising a transgene encoding a polypeptide comprising an immunoglobulin heavy chain variable region, wherein the transgene comprises (1) a plurality of immunoglobulin heavy chain variable (V) exons encoding human immunoglobulin heavy chain V polypeptides;
- (2) non-coding sequences between the V exons;
(3) a plurality of immunoglobulin heavy chain joining (J) coding sequences encoding human immunoglobulin heavy chain joining J polypeptides; and
(4) non-coding sequences between the J coding sequences;
wherein the non-coding sequences between the V exons and the non-coding sequences between the J coding sequences are derived from mouse, rat, or a combination thereof immunoglobulin heavy chain non-coding sequences. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- (2) non-coding sequences between the V exons;
Specification