Purification of nucleic acids using metal-titanium oxides

• US 10,526,596 B2
• Filed: 07/05/2019
• Issued: 01/07/2020
• Est. Priority Date: 07/14/2015
• Status: Active Grant

• Current Assignee: Abbott Molecular Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Molecular Incorporated (Abbott Laboratories Incorporated)
• Inventors: Gundling, Gerard J.
• Primary Examiner(s): Horlick, Kenneth R
• Application Number:
  US16/503,874
• Publication Number:
  US 20190345481A1
• Time in Patent Office: 186 Days
• Field of Search: None
• CPC Class Codes: C01G 23/00 Compounds of titanium preparation of Ti-compounds from ores or scraps C22B34/12
• CPC Class Codes: C01P 2004/61 Micrometer sized, i.e. from 1-100 micrometer
• CPC Class Codes: C01P 2004/62 Submicrometer sized, i.e. from 0.1-1 micrometer
• CPC Class Codes: C07H 1/08 from natural products
• CPC Class Codes: C12N 15/1006 by means of a solid support carrier, e.g. particles, polymers

Abstract

The present disclosure relates to systems and methods for purifying nucleic acid. In particular, the present disclosure relates to systems and methods for purifying nucleic acids using metal or metal oxide compositions.

Claims

1. A method of capturing RNA and/or DNA from a biological sample, comprising:

a) contacting said sample with a particle or solid surface comprising or coated with a metal oxide such that DNA and/or RNA in said sample binds said particle wherein said metal oxide is selected from the group consisting of AlTi, CaTi, CoTi, Fe₂Ti, Fe₃Ti, MgTi, MnTi, NiTi, SnTi and ZnTi;

b) washing said particle or solid surface to remove contaminants; and

c) eluting RNA and/or DNA from said particle or solid surface.

2. The method of claim 1, wherein said metal oxide is an anhydride or hydrated form of said metal oxide.

3. The method of claim 1, wherein said particle has a diameter of 0.5 to 50 μ

m.

4. The method of claim 1, wherein said particle or solid surface is comprised of a polymer, a magnetic material, a metallic material, an inorganic solid, or a combination thereof.

5. The method of claim 4, wherein said inorganic solid is silica.

6. The method of claim 1, wherein said solid surface has a shape selected from the group consisting of planer, acicular, cuboidal, tubular, fibrous, columnar, and amorphous.

7. The method of claim 1, wherein said eluting comprises an elution buffer.

8. The method of claim 7, wherein said elution buffer comprises phosphate.

9. The method of claim 8, wherein said phosphate is an inorganic or an organophosphate.

10. The method of claim 8, wherein said phosphate is present in said elution buffer at a concentration of 0.5 to 20 mM.

11. The method of claim 1, wherein said RNA and/or DNA is eukaryotic, prokaryotic or viral RNA.

12. The method of claim 11, wherein said DNA and/or RNA is from a eukaryotic, prokaryotic or viral pathogen.

13. The method of claim 1, wherein said particle or solid surface preferentially binds RNA or DNA.

14. The method of claim 1, further comprising the step of determining the identity and/or amount of said DNA and/or RNA present in said sample.

15. The method of claim 14, wherein said determining comprises the use of one or more detection methods selected from the group consisting of amplification, hybridization, and sequencing.