Meniscus reducing member

• US 10,537,891 B2
• Filed: 03/12/2018
• Issued: 01/21/2020
• Est. Priority Date: 01/10/2013
• Status: Active Grant

• Current Assignee: Stemcell Technologies Inc. (Stemcell Technologies Canada Inc.)
• Original Assignee: Stemcell Technologies Inc. (Stemcell Technologies Canada Inc.)
• Inventors: Egeler, Oliver, Woodside, Steven
• Primary Examiner(s): Krcha, Matthew D
• Application Number:
  US15/918,798
• Publication Number:
  US 20180200711A1
• Time in Patent Office: 680 Days
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2300/166 Suprahydrophobic; Ultraphobic; Lotus-effect
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C12M 1/00 Apparatus for enzymology or microbiology
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

Claims

1. A vessel comprising:

a) a closed bottom wall and a sidewall extending from the bottom wall to an open top portion for retaining a volume of liquid within the vessel, the sidewall comprising an interior surface;

b) a physical surface feature overlying at least a portion of the interior surface to engage a free surface of a liquid in the vessel, the physical surface feature including a first inner surface, a second inner surface below the first inner surface when the liquid is contained in the vessel, and a third surface extending between the first and second surfaces, the third surface intersecting both the first and second inner surfaces, the first inner surface, second inner surface and third surface configured to physically alter a receding contact angle between the liquid and the physical surface feature;

c) a step edge defined by the intersection of the second inner surface and the third inner surface, the step edge configured to engage the free surface of the liquid in the vessel; and

d) a hydrophobic or superhydrophobic coating material applied to the first inner surface, wherein the second inner surface is substantially free from the coating material, and whereby the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is between about 90 degrees and less than 180 degrees.

2. The vessel of claim 1, wherein the physical surface feature is disposed on a separate insert member configured to be received within the vessel.

3. The vessel of claim 1, wherein the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is closer to 180 degrees than a receding contact angle formed between the liquid and the physical surface feature in the absence of the coating material.

4. The vessel of claim 1, wherein the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is closer to 180 degrees than an unaltered receding contact angle measured between the liquid and a portion of the interior surface of the vessel.

5. The vessel of claim 1, wherein the coating material comprises at least one of i) a solution containing at least 63% by weight of one or more of methyl perfluorobutylethyl siloxane, methylhydrogen siloxane, dimethyl siloxane and methyl vinyl siloxane crosslinked with a crosslinking solution that comprises at least 60% by weight of one or more of trimethylsiloxy-terminated methyl(perfluorobutylethyl) siloxane, and trimethylsiloxy-terminated methylhydrogen siloxane, or at least one of ii) a coating of a polymer of siloxane, a siloxane based coating, a perfluorocarbon based coating and a nano-particle based coating.

6. The vessel of claim 1, wherein the physical surface feature is integral with the sidewall of the vessel.

7. The vessel of claim 1, wherein the physical surface feature extends continuously around an inner perimeter of the vessel.

8. The vessel of claim 1, wherein the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is between about 110 degrees and about 160 degrees.

9. The vessel of claim 8, wherein the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is between about 120 degrees and about 150 degrees.

10. The vessel of claim 1, wherein the coating material is superhydrophobic.

11. The vessel of claim 1, wherein the receding contact angle formed between the liquid and the first inner surface of the physical surface feature is closer to 180 degrees than an intrinsic contact angle formed between the liquid and the physical surface feature.

12. The vessel of claim 1, wherein the third surface is generally perpendicular to both the first and second inner surfaces or is inclined at an oblique angle to both the first and second inner surfaces.

13. The vessel of claim 1, wherein the second inner surface is laterally inwardly offset from the first inner surface.

14. The vessel of claim 1, wherein the third surface is curved.

15. The vessel of claim 1, wherein when the liquid is in the vessel the free surface is pinned at a level of the step edge.