Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells

• US 10,542,734 B2
• Filed: 01/15/2019
• Issued: 01/28/2020
• Est. Priority Date: 06/06/2012
• Status: Active Grant

• Current Assignee: Inguran LLC
• Original Assignee: Inguran LLC
• Inventors: Moreno, Juan, Bevier, Gregg, Dobrinsky, John
• Primary Examiner(s): Noble, Marcia S
• Application Number:
  US16/248,569
• Publication Number:
  US 20190141965A1
• Time in Patent Office: 378 Days
• CPC Class Codes: A01K 2227/108 Swine
• CPC Class Codes: A01K 67/02 Breeding vertebrates
• CPC Class Codes: A61B 1/015 Control of fluid supply or evacuation
• CPC Class Codes: A61B 1/04 combined with photographic or television appliances
• CPC Class Codes: A61B 1/303 for the vagina, i.e. vaginoscopes
• CPC Class Codes: A61B 1/313 for introducing through surgical openings, e.g. laparoscopes
• CPC Class Codes: A61B 10/0012 Ovulation-period determination for animals A61D17/002
• CPC Class Codes: A61B 2034/2057 Details of tracking cameras
• CPC Class Codes: A61B 34/20 Surgical navigation systems; Devices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis
• CPC Class Codes: A61B 8/08 Detecting organic movements or changes, e.g. tumours, cysts, swellings A61B8/02 - A61B8/06 take precedence
• CPC Class Codes: A61D 19/027 Devices for injecting semen into animals, e.g. syringes, guns, probes
• CPC Class Codes: A61M 31/002 Devices for releasing a drug at a continuous and controlled rate for a prolonged period of time artificial gland structures or devices A61F2/022; intra-uterine contraceptive devices A61F6/14; tampons for introducing into the vagina A61F13/20, A61L15/00; suppositories or bougies for intra-vaginal or intra-uterine application A61K9/02; physical forms of medicinal preparations for sustained or differential drug release A61K9/20, A61K9/50
• CPC Class Codes: G16B 20/00 ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
• CPC Class Codes: G16B 20/40 Population genetics; Linkage disequilibrium

Abstract

The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.

Claims

1. A method of increasing the genetic progress of a line or breed of swine within a genetic nucleus comprising the steps of:

providing a boar from a genetic nucleus and a sow from a genetic nucleus, wherein said boar and said sow are from a line or breed within said genetic nucleus and wherein said line or breed of said boar and said sow are the same line or breed;

collecting a semen sample from said boar;

sorting said semen sample into at least two subpopulations of sperm cells, wherein at least 80% of a first subpopulation bears X-chromosomes or Y-chromosomes;

inseminating said sow with sperm cells from said first subpopulation to produce offspring from said sow;

genotyping said offspring to identify one or more genetic markers;

selecting one or more of said offspring as a parent in the line or breed in the genetic nucleus based on the one or more identified genetic markers.

2. The method of claim 1 wherein said line or breed is a gilt line and said first subpopulation bears X-chromosomes.

3. The method of claim 1 wherein said line or breed is a boar line and said first subpopulation bears Y-chromosomes.

4. The method of claim 1, wherein the one or more genetic markers are single nucleotide polymorphisms.

5. The method of claim 4, wherein the single nucleotide polymorphisms are correlated with feed efficiency, average daily gain or carcass lean.