Hydrodynamic focusing apparatus and methods

  • US 10,583,439 B2
  • Filed: 03/14/2014
  • Issued: 03/10/2020
  • Est. Priority Date: 03/14/2013
  • Status: Active Grant
First Claim
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1. A microfluidic assembly for use with a particle processing instrument, the microfluidic assembly comprising:

  • a substrate; and

    a flow channel formed in the substrate, the flow channel having;

    an inlet configured to receive a sample stream;

    a fluid focusing region configured to focus the sample stream, the fluid focusing region having a lateral fluid focusing feature, a first vertical fluid focusing feature, and a second vertical fluid focusing feature, the lateral, the first vertical, and the second vertical fluid focusing features provided at different longitudinal locations along the flow channel, wherein a bottom surface of the flow channel lies in a first plane upstream of the first and second vertical fluid focusing features and the bottom surface of the flow channel shifts vertically upward to lie in a second plane downstream of the first and second vertical focusing features; and

    an inspection region at least partially downstream of the fluid focusing region.

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