Compositions and methods for treating and preventing tissue injury and disease

• US 10,596,202 B2
• Filed: 01/08/2018
• Issued: 03/24/2020
• Est. Priority Date: 09/19/2012
• Status: Active Grant

• Current Assignee: Microvascular Tissues, Inc.
• Original Assignee: Microvascular Tissues, Inc.
• Inventors: Peterson, Dale R., Mattern, Ralph-Heiko, Ohashi, Kevin L., Pickett, Lael J., Gong, Glen, El-Kalay, Mohammad A.
• Primary Examiner(s): Mi, Qiuwen
• Application Number:
  US15/865,220
• Publication Number:
  US 20180161379A1
• Time in Patent Office: 806 Days
• Field of Search: None
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/44 Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
• CPC Class Codes: A61K 35/545 Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
• CPC Class Codes: A61L 2300/41 Anti-inflammatory agents, e.g. NSAIDs
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2400/06 Flowable or injectable implant compositions
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 2430/32 for nerve reconstruction
• CPC Class Codes: A61L 2430/34 for soft tissue reconstruction
• CPC Class Codes: A61L 2430/38 for reconstruction of the spine, vertebrae or intervertebral discs
• CPC Class Codes: A61L 27/3625 Vascular tissue, e.g. heart valves
• CPC Class Codes: A61L 27/3687 characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3878 Nerve tissue, brain, spinal cord, nerves, dura mater
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue

Abstract

The present invention provides novel compositions comprising multipotent cells or microvascular tissue, wherein the cells or tissue has been sterilized and/or treated to inactivated viruses, and related methods of using these compositions to treat or prevent tissue injury or disease in an allogeneic subject.

Claims

1. A method for the treatment of nerve damage, comprising administering a therapeutic agent to a subject in need thereof, wherein the therapeutic agent comprises enriched, nonviable multipotent cells, wherein the nerve damage comprises peripheral neuropathy.

2. The method of claim 1, wherein the multipotent cells are sterilized by irradiation.

3. The method of claim 1, wherein the multipotent cells have been isolated from microvascular tissue.

4. The method of claim 1, wherein the multipotent cells have been enriched by removal of extraneous tissue and culturing the multipotent cells.

5. The method of claim 1, wherein the multipotent cells have been enriched by culturing the multipotent cells.

6. The method of claim 1, wherein administering the therapeutic agent comprises injection or surgical application to the region of damaged nerves.

7. The method of claim 1, wherein administering the therapeutic agent comprises systemic injection.

8. The method of claim 1, wherein the therapeutic agent is a powder, gel or ointment applied topically near the site of nerve damage.