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Pichia pastoris strains for producing predominantly homogeneous glycan structure

  • US 10,612,033 B2
  • Filed: 05/07/2019
  • Issued: 04/07/2020
  • Est. Priority Date: 10/23/2012
  • Status: Active Grant
First Claim
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1. An engineered strain of Pichia pastoris, comprising:

  • a mutant OCH1 allele which is transcribed into a mRNA coding for a mutant OCH1 protein,wherein said mutant OCH1 protein comprises a catalytic domain that (i) comprises residues 45-404 of the wild type OCH1 protein of the amino acid sequence of SEQ ID NO;

    2, or (ii) is at least 90% identical to the amino acids corresponding to residues 45-404 of the wild type OCH1 protein of SEQ ID NO;

    2 and has α

    -1, 6-mannosyltransferase activity;

    wherein said mutant OCH1 protein lacks an N-terminal sequence for targeting the mutant OCH1 protein to the Golgi apparatus; and

    wherein said engineered strain is deficient in a protease.

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