Spatially encoded biological assays
First Claim
Patent Images
1. A method for determining a location of a nucleic acid in a tissue sample comprising:
- (a) providing sections of the tissue sample;
(b) obtaining an image of a section of the tissue sample in (a) to identify a region of interest in the sections of the tissue sample;
(c) delivering a probe for the nucleic acid to the identified region of interest in a section of the tissue sample in (a), where the probe contains a probe region that selectively binds to the nucleic acid and a coding tag that identifies a site in the identified region of interest to which the probe was delivered; and
(d) determining all or a portion of a sequence of the probe selectively bound to the nucleic acid or a complement thereof, and using the determined sequence to determine the location of the nucleic acid in the tissue sample.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
273 Citations
30 Claims
-
1. A method for determining a location of a nucleic acid in a tissue sample comprising:
-
(a) providing sections of the tissue sample; (b) obtaining an image of a section of the tissue sample in (a) to identify a region of interest in the sections of the tissue sample; (c) delivering a probe for the nucleic acid to the identified region of interest in a section of the tissue sample in (a), where the probe contains a probe region that selectively binds to the nucleic acid and a coding tag that identifies a site in the identified region of interest to which the probe was delivered; and (d) determining all or a portion of a sequence of the probe selectively bound to the nucleic acid or a complement thereof, and using the determined sequence to determine the location of the nucleic acid in the tissue sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
-
Specification