Polysaccharide-polyamine copolymer and use thereof in reducing uric acid concentration in plasma

• US 10,639,325 B2
• Filed: 03/18/2016
• Issued: 05/05/2020
• Est. Priority Date: 03/20/2015
• Status: Active Grant

• Current Assignee: Dazhi Yang
• Original Assignee: Dazhi Yang
• Inventors: Yang, Dazhi
• Primary Examiner(s): Falkowitz, Anna R
• Application Number:
  US15/559,196
• Publication Number:
  US 20180110801A1
• Time in Patent Office: 1,509 Days
• CPC Class Codes: A61K 31/738 Cross-linked polysaccharides
• CPC Class Codes: A61K 31/785 Polymers containing nitrogen
• CPC Class Codes: A61K 9/14 Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles microspheres A61K9/16; microcapsules A61K9/50; nanocapsules, nanoparticles of the matrix type A61K9/51
• CPC Class Codes: A61P 7/08 Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock artificial tears A61P27/04
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/267 Cross-linked polymers
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: B01J 20/28085 being more than 50 nm, i.e. macropores
• CPC Class Codes: B01J 20/3085 Chemical treatments not covered by groups B01J20/3007 - B01J20/3078
• CPC Class Codes: C08B 15/06 containing nitrogen , e.g. carbamates
• CPC Class Codes: C08F 251/02 on to cellulose or derivatives thereof
• CPC Class Codes: C08G 2101/00 Manufacture of cellular products
• CPC Class Codes: C08G 2210/00 Compositions for preparing hydrogels
• CPC Class Codes: C08G 73/0206 Polyalkylene(poly)amines
• CPC Class Codes: C08G 81/00 Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers involving only carbon-to-carbon unsaturated bond reactions C08F299/00 ; polyester-amides C08G69/44; polyester-imides C08G73/16; polyamides-imides C08G73/14; block- or graft polymers containing polysiloxane sequences C08G77/42
• CPC Class Codes: C08G 83/002 Dendritic macromolecules
• CPC Class Codes: C08J 2205/022 Hydrogel, i.e. a gel containing an aqueous composition
• CPC Class Codes: C08J 2207/10 Medical applications, e.g. biocompatible scaffolds
• CPC Class Codes: C08J 2387/00 Characterised by the use of unspecified macromolecular compounds, obtained otherwise than by polymerisation reactions only involving unsaturated carbon-to-carbon bonds
• CPC Class Codes: C08J 9/16 : Making expandable particles
• CPC Class Codes: C08L 1/08 : Cellulose derivatives
• CPC Class Codes: C08L 3/20 : Oxidised amylose; Oxidised amylopectin
• CPC Class Codes: C08L 5/02 : Dextran; Derivatives thereof
• CPC Class Codes: C08L 5/08 : Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof

Abstract

Disclosed is a pharmaceutical composition for treating hyperuricemia (HUA). The pharmaceutical composition includes a polysaccharide-polyamine copolymer and a pharmaceutically acceptable salt thereof as active ingredients. The polysaccharide-polyamine copolymer is formed by copolymerization of the following two parts: a selectively oxidized polysaccharide with 2,3-dialdehydo, and a polyamine with an amino functional group; the polyamine with an amino functional group and the selectively oxidized polysaccharide with 2,3-dialdehydo can form a net structure by means of covalent crosslinking, resulting in a hydrogel with an amino functional group or a granular polysaccharide-polyamine copolymer, wherein the amino functional group in the hydrogel with an amino functional group or the granular polysaccharide-polyamine copolymer can be protonated so as to form a cationic copolymer of a three-dimensional network structure having a protonated site, and the nitrogen content of the cationic copolymer and the nitrogen content of the polysaccharide-polyamine copolymer are above 12.3 wt %, and both the cationic copolymer and the polysaccharide-polyamine copolymer are water-insoluble.

Claims

1. A pharmaceutical composition for treatments of hyperuricemia, the pharmaceutical composition comprises an amino-polysaccharide copolymer and a pharmaceutically acceptable salt thereof as an active ingredient, the amino-polysaccharide copolymer is a copolymerization product of the following two parts:

a selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, and an amino polymer which provides an amino functionality;

the amino polymer cross linking the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety to form a net-like structure and finally to provide a hydrogel having an amino functionality or a particulate amino-polysaccharide copolymer, amino functionalities of the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer can be protonated to form a cationic copolymer having three dimensional network structure and protonation sites, a nitrogen content of the cationic copolymer or the amino-polysaccharide copolymer is at least 12.3% weight percent, or at least 15% weight percent, or at least 22% weight percent, or at least 40% weight percent, the cationic copolymer and the amino-polysaccharide copolymer are both insoluble in water,

wherein in the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, a ratio of an amount of oxidized glucose units to the total amount of glucose units is at least 50%, at least 70%, or at least 80%.

2. The pharmaceutical composition according to claim 1, wherein a nitrogen content of the amino polymer which provides an amino functionality is at least 24.5% weight percent, or at least 30% weight percent, or at least 44% weight percent, or at least 80% weight percent.

3. The pharmaceutical composition according to claim 1, wherein a molecular weight of the amino polymer which provides an amino functionality is from about 15,000 to about 900,000.

4. The pharmaceutical composition according to claim 1, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety is one or more selected from the group consisting of selectively oxidized cellulose, selectively oxidized amylopectin, and selectively oxidized chitosan.

5. The pharmaceutical composition according to claim 1, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety has at least one of a β

-1,4-glycosidic bond or a β

-1,6-glycosidic bond.

6. The pharmaceutical composition according to claim 5, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety has a β

-1,4-glycosidic bond and does not have a β

-1,6-glycosidic bond.

7. The pharmaceutical composition according to claim 1, wherein the amino polymer which provides an amino functionality is one or more selected from the group consisting of polyethyleneimine (PEI), poly(allylamine), polypropyleneimine (PPI), and polypropylenimine tetramine.

8. The pharmaceutical composition according to claim 1, wherein the amino polymer which provides an amino functionality has a branched structure or a dendritic structure.

9. The pharmaceutical composition according to claim 1, wherein a size of the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer or the cationic copolymer is from 30 μ

m to 10 mm, or from 100 μ

m to 10 mm, or from 300 μ

m to 5 mm.

10. The pharmaceutical composition according to claim 1, wherein the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer or the cationic copolymer has pores with diameters less than 50 μ

m, or has pores with diameters from 100 nm to 50 μ

m, or has pores with diameters from 200 nm to 40 μ

m, or has pores with diameters from 300 nm to 30 μ

m, or has pores with diameters from 400 nm to 20 μ

m, or has pores with diameters from 500 nm to 10 μ

m, or has pores with diameters from 800 nm to 5 μ

m.

11. A method for reducing serum uric acid comprising administering a pharmaceutical composition comprising an amino-polysaccharide copolymer and a pharmaceutically acceptable salt thereof as an active ingredient, wherein, the amino-polysaccharide copolymer is a copolymerization product of the following two parts:

a selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, and an amino polymer which provides an amino functionality;

the amino polymer cross linking the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety to form a net-like structure and finally to provide a hydrogel having an amino functionality or a particulate amino-polysaccharide copolymer, amino functionalities of the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer can be protonated to form a cationic copolymer having three dimensional network structure and protonation sites, a nitrogen content of the cationic copolymer or the amino-polysaccharide copolymer is at least 12.3% weight percent, or at least 15% weight percent, or at least 22% weight percent, or at least 40% weight percent, the cationic copolymer and the amino-polysaccharide copolymer are both insoluble in water;

wherein in the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, a ratio of an amount of oxidized glucose units to the total amount of glucose units is at least 50%, at least 70%, or at least 80%.

12. The method according to claim 11, wherein a nitrogen content of the amino polymer which provides an amino functionality is at least 24.5% weight percent, or at least 30% weight percent, or at least 44% weight percent, or at least 80% weight percent.

13. The method according to claim 11, wherein a molecular weight of the amino polymer which provides an amino functionality is from about 15,000 to about 900,000.

14. The method according to claim 11, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety is one or more selected from the group consisting of selectively oxidized cellulose, selectively oxidized amylopectin, and selectively oxidized chitosan.

15. The method according to claim 11, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety has at least one of a β

-1,4-glycosidic bond or a β

-1,6-glycosidic bond.

16. The method according to claim 15, wherein the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety has a β

-1,4-glycosidic bond and does not have a β

-1,6-glycosidic bond.

17. The method according to claim 11, wherein the amino polymer which provides an amino functionality is one or more selected from the group consisting of polyethyleneimine (PEI), poly(allylamine), polypropyleneimine (PPI), and polypropylenimine tetramine.

18. The method according to claim 11, wherein the amino polymer which provides an amino functionality has a branched structure or a dendritic structure.

19. The method according to claim 11, wherein a size of the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer or the cationic copolymer is from 30 μ

m to 10 mm, or from 100 μ

m to 10 mm, or from 300 μ

m to 5 mm.

20. The method according to claim 11, wherein the hydrogel having an amino functionality or the particulate amino-polysaccharide copolymer or the cationic copolymer has pores with diameters less than 50 μ

m, or has pores with diameters from 100 nm to 50 μ

m, or has pores with diameters from 200 nm to 40 μ

m, or has pores with diameters from 300 nm to 30 μ

m, or has pores with diameters from 400 nm to 20 μ

m, or has pores with diameters from 500 nm to 10 μ

m, or has pores with diameters from 800 nm to 5 μ

m.