Spatially encoded biological assays
DCFirst Claim
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1. A method for determining the presence or abundance of a nucleic acid at a location in a tissue section comprising:
- (a) contacting the tissue section with an array comprising a plurality of features, wherein a feature of the plurality of features comprises a capture agent, wherein the capture agent comprises;
(i) a first sequence that specifically binds the nucleic acid, and(ii) a second sequence comprising a coding tag, wherein a sequence of the coding tag corresponds to a location of the capture agent on the array;
(b) determining all or a portion of the sequence of the coding tag, or a complement thereof, and all or a portion of the sequence of the nucleic acid specifically bound to the capture agent, or a complement thereof, and using the determined sequences to determine presence or abundance of the nucleic acid at a location in the tissue section.
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Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
273 Citations
30 Claims
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1. A method for determining the presence or abundance of a nucleic acid at a location in a tissue section comprising:
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(a) contacting the tissue section with an array comprising a plurality of features, wherein a feature of the plurality of features comprises a capture agent, wherein the capture agent comprises; (i) a first sequence that specifically binds the nucleic acid, and (ii) a second sequence comprising a coding tag, wherein a sequence of the coding tag corresponds to a location of the capture agent on the array; (b) determining all or a portion of the sequence of the coding tag, or a complement thereof, and all or a portion of the sequence of the nucleic acid specifically bound to the capture agent, or a complement thereof, and using the determined sequences to determine presence or abundance of the nucleic acid at a location in the tissue section. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification