Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses therefor

  • US 10,695,441 B2
  • Filed: 10/02/2018
  • Issued: 06/30/2020
  • Est. Priority Date: 09/30/2003
  • Status: Active Grant
First Claim
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1. A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp1 capsid protein having a sequence comprising amino acids 1 to 736 of SEQ ID NO:

  • 123 (AAV9) or a sequence at least 95% identical to the full length of amino acids 1 to 736 of SEQ ID NO;

    123, wherein the vp1 protein having at least 95% identity has up to 37 amino acid modifications in the amino acids 1 to 736 limited to an amino acid position of SEQ ID NO;

    123 selected from amino acids;

    1, 2, 3, 4, 5, 6, 7, 12, 13, 14, 15, 16, 19, 21, 22, 23, 24, 26, 27, 29, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 48, 50, 51, 56, 60, 61, 66, 67, 70, 71, 73, 77, 78, 80, 81, 84, 85, 90, 91, 92, 94, 96, 97, 100, 102, 103, 105, 106, 109, 116, 125, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 141, 142, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 209, 210, 211, 212, 213, 214, 215, 216, 223, 224, 227, 228, 229, 233, 234, 235, 236, 237, 238, 240, 241, 242, 246, 248, 249, 251, 252, 256, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 275, 277, 278, 283, 284, 291, 292, 293, 298, 301, 302, 304, 305, 306, 308, 311, 312, 313, 314, 315, 317, 323, 324, 327, 328, 329, 330, 331, 332, 334, 337, 339, 342, 344, 346, 348, 349, 351, 352, 356, 357, 358, 359, 360, 361, 363, 364, 366, 369, 370, 373, 374, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 392, 393, 394, 395, 397, 398, 399, 403, 404, 405, 411, 412, 413, 414, 416, 419, 422, 425, 426, 427, 428, 429, 431, 432, 433, 434, 436, 440, 441, 445, 446, 447, 448, 449, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 478, 479, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 512, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 562, 564, 566, 567, 568, 569, 571, 572, 573, 574, 575, 576, 577, 579, 580, 581, 582, 583, 584, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 605, 607, 608, 609, 612, 613, 614, 615, 618, 624, 625, 626, 627, 628, 634, 635, 638, 640, 642, 646, 647, 648, 651, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 670, 672, 675, 676, 678, 680, 681, 682, 683, 684, 687, 688, 691, 694, 695, 696, 699, 701, 703, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 720, 722, 723, 724, 725, 726, 729, 732, 734 and 735, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence, and wherein the recombinant nucleic acid molecule is a plasmid.

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