Methods for assaying cellular binding interactions

  • US 10,746,737 B2
  • Filed: 03/12/2020
  • Issued: 08/18/2020
  • Est. Priority Date: 07/16/2010
  • Status: Active Grant
First Claim
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1. A method of assaying for a binding interaction between a secreted monoclonal antibody produced by a single antibody producing cell (APC) and an antigen, the method comprising:

  • retaining the single APC within a chamber having a volume of from 100 pL to 100 nL, a solid wall, and an aperture that defines an opening of the chamber;

    incubating the single APC within the chamber to produce a secreted monoclonal antibody;

    exposing the secreted monoclonal antibody to a first removeable capture substrate, wherein the first removeable capture substrate is in fluid communication with the secreted monoclonal antibody and wherein the first removeable capture substrate is capable of binding the secreted monoclonal antibody;

    incubating the secreted monoclonal antibody with the removeable capture substrate to produce a bound antibody;

    bringing a first fluid volume comprising the antigen in fluid communication with the bound antibody;

    measuring a binding interaction between the bound antibody and the antigen; and

    lysing the single APC and capturing the nucleic acids of the single APC on a second removeable capture substrate.

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