Porous mesh spectrometry methods and apparatus

• US 10,760,965 B2
• Filed: 09/20/2018
• Issued: 09/01/2020
• Est. Priority Date: 03/21/2016
• Status: Active Grant

• Current Assignee: COR Health Incorporated
• Original Assignee: Nueon Inc.
• Inventors: Messerschmidt, Robert G., Jones, Howland D. T.
• Primary Examiner(s): Bologna, Dominic J
• Application Number:
  US16/137,012
• Publication Number:
  US 20190226910A1
• Time in Patent Office: 712 Days
• CPC Class Codes: G01J 3/0264 Electrical interface; User interface
• CPC Class Codes: G01J 3/0267 Sample holders for colorimetry
• CPC Class Codes: G01N 2021/0339 Holders for solids, powders
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/035 Supports for sample drops
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/25 Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

Described herein are methods and apparatus for spectroscopic analysis of samples. In many embodiments, an apparatus for providing spectroscopic analysis of a sample comprises a sample holder. For example, the sample holder may comprise a consumable single use sample holder that can be readily coupled to and removed from a measurement apparatus such as a spectrometer. The sample holder may comprise a measurement surface configured to receive the sample during measurement, wherein the measurement surface may comprise a porous mesh. The porous mesh can receive the sample to optimally configure the sample for spectroscopic measurement, as described in further detail herein.

Claims

1. A method of measuring spectra of a sample, the method comprising:

adding an internal standard to the sample at a known concentration;

depositing, after adding the internal standard to the sample, the sample onto a measurement surface of an optical waveguide, the measurement surface having a porous mesh disposed thereover, and the porous mesh comprising a plurality of pores to receive a portion of the sample therein; and

measuring the portion of the sample disposed within the plurality of pores and in contact with the measurement surface with an evanescent wave generated at the measurement surface from total internal reflection of measurement light within the optical waveguide.

2. A method as in claim 1, further comprising drying the sample deposited onto the measurement surface and the porous mesh until the sample is at least partially dehydrated.

3. A method as in claim 2, wherein the sample the dried until the sample comprises about 0% water by weight.

4. A method as in claim 2, wherein the measuring comprises measuring the portion of the sample at a plurality of time points during the drying of the sample.

5. A method as in claim 1, further comprising determining a volume of the sample disposed within the plurality of pores, based on a measured absorbance of a sample component having a known spectral signal, a molar absorptivity of the sample component, and a concentration of the sample component.

6. A method as in claim 5, further comprising collecting the sample with a sample collection device comprising the internal standard impregnated therein, wherein the impregnated internal standard dissolves into the sample to the known concentration when the sample is collected with the sample collection device.

7. A method as in claim 1, further comprising transmitting a measurement signal to a remote server for analysis of the measurement signal to produce analyzed spectral data of the sample.

8. A method as in claim 7, further comprising transmitting the analyzed spectral data of the sample to a personal computing device.

9. A method as in claim 1, wherein the depositing further comprises depositing the sample onto one or more absorbent members in contact with the porous mesh, and wherein the method further comprises sending the one or more absorbent members having absorbed sampled to a central laboratory for additional analysis of the sample.

10. A method as in claim 9, further comprising identifying the one or more absorbent members via one or more unique identifiers of the one or more absorbent members.

11. A method as in claim 9, further comprising transmitting results of the additional analysis to a personal computing device.

12. A method as in claim 1, wherein each of the plurality of pores has a diameter of about 0.1 μ

m to about 20 μ

m.

13. A method as in claim 12, wherein each of the plurality of pores has a diameter of at least about 10 μ

m.

14. A method as in claim 12, wherein each of the plurality of pores has a diameter of at least about 5 μ

m.

15. A method as in claim 12, wherein each of the plurality of pores has a diameter of at least about 1 μ

m.

16. A method as in claim 1, wherein each of the plurality of pores is sized and shaped to selectively receive a first portion of the sample and selectively exclude a second portion of the sample.

17. A method as in claim 1, wherein the sample comprises whole blood, and wherein the portion of the sample comprises one or more components of the whole blood.

18. A method as in claim 17, wherein the portion of the sample comprises plasma, and does not include cellular components.

19. A method as in claim 17, wherein the portion of the sample comprises plasma and red blood cells, and does not include white blood cells.

20. A method as in claim 17, wherein the portion of the sample comprises plasma, red blood cells, and white blood cells.