Heterodimerized polypeptide

  • US 10,766,960 B2
  • Filed: 12/26/2013
  • Issued: 09/08/2020
  • Est. Priority Date: 12/27/2012
  • Status: Active Grant
First Claim
Patent Images

1. A heterodimer comprising a first polypeptide and a second polypeptide,wherein the first polypeptide comprises an IgG Fc region comprising a set of amino acids selected from the group consisting of sets (a) to (aj) below (all positions by EU numbering):

  • (a) amino acid E at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298;

    amino acid D at position 327;

    (b) amino acid S at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (c) amino acid L at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (d) amino acid L at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (e) amino acid S at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (f) amino acid F at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (g) amino acid E at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (h) amino acid F at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (i) amino acid V at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (j) amino acid D at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (k) amino acid Q at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (l) amino acid I at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (m) amino acid M at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (n) amino acid T at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (o) amino acid A at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (p) amino acid G at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (q) amino acid H at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (r) amino acid V at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (s) amino acid D at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (t) amino acid Q at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (u) amino acid I at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (v) amino acid M at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (w) amino acid T at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (x) amino acid A at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (y) amino acid G at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (z) amino acid H at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid M at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (aa) amino acid F at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (ab) amino acid E at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (ac) amino acid D at position 234;

    amino acid Q at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid E at position 270; and

    amino acid A at position 298;

    (ad) amino acid V at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (ae) amino acid I at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (af) amino acid L at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (ag) amino acid E at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (ah) amino acid D at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327;

    (ai) amino acid F at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327; and

    (aj) amino acid T at position 234;

    amino acid Y at position 235;

    amino acid W at position 236;

    amino acid I at position 239;

    amino acid D at position 268;

    amino acid A at position 298; and

    amino acid D at position 327; and

    the second polypeptide comprises an IgG Fc region that is different from the Fc region of the first polypeptide and comprises a set of amino acids selected from the group consisting of sets (ak) to (aq) (all positions by EU numbering);

    (ak) amino acid E at position 270;

    amino acid D at position 326;

    amino acid K at position 330; and

    amino acid E at position 334;

    (al) amino acid E at position 270;

    amino acid D at position 326;

    amino acid M at position 330; and

    amino acid E at position 334;

    (am) amino acid E at position 270;

    amino acid D at position 326;

    amino acid A at position 330; and

    amino acid E at position 334;

    (an) amino acid E at position 270;

    amino acid D at position 326;

    amino acid F at position 330; and

    amino acid E at position 334;

    (ao) amino acid E at position 270;

    amino acid D at position 326;

    amino acid I at position 330; and

    amino acid E at position 334;

    (ap) amino acid E at position 270;

    amino acid D at position 326;

    amino acid Y at position 330; and

    amino acid E at position 334; and

    (aq) amino acid E at position 270;

    amino acid D at position 326;

    amino acid H at position 330; and

    amino acid E at position 334.

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