Stabilizing compositions and methods for extraction of ribonucleic acid

  • US 10,767,215 B2
  • Filed: 05/15/2018
  • Issued: 09/08/2020
  • Est. Priority Date: 10/06/2006
  • Status: Active Grant
First Claim
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1. A phenol-free method for preserving ribonucleic acid from a biological sample comprising the steps of:

  • a. obtaining the sample from a subject;

    b. contacting the sample with a composition comprising an anionic detergent, wherein the anionic detergent is at a concentration of 1% to 8%, and a buffering agent at a pH of about 5 to about 8.2 to form a mixture; and

    c. storing the mixture at room temperature;

    wherein the composition stabilizes the ribonucleic acid at room temperature.

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