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Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same

  • US 10,851,398 B2
  • Filed: 09/28/2018
  • Issued: 12/01/2020
  • Est. Priority Date: 03/25/2005
  • Status: Active Grant
First Claim
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1. A method for producing a recombinant glucose dehydrogenase (GLD) comprising:

  • (i) contacting a transformed fungal or yeast host cell with a liquid culture medium in a fermenter, wherein the host cell comprises a recombinant polynucleotide encoding an Aspergillus species flavin adenine dinucleotide-linked GLD comprising the sequences set forth in SEQ ID NOS;

    8 to 12;

    (ii) cultivating the host cell and recombinantly expressing the GLD; and

    (iii) obtaining recombinantly-expressed GLD from the host cell.

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