Adsorbing material for multiple pathogenic factors of sepsis as well as preparation method and application thereof

• US 10,898,632 B2
• Filed: 10/27/2016
• Issued: 01/26/2021
• Est. Priority Date: 10/29/2015
• Status: Active Grant

• Current Assignee: Chongqing Zhengbo Biotechnology Co., Ltd.
• Original Assignee: Chongqing Zhengbo Biotechnology Co., Ltd.
• Inventors: Zheng, Yue, Deng, Jun, Liu, Qingguang
• Primary Examiner(s): Witherspoon, Sikarl A
• Application Number:
  US15/770,629
• Publication Number:
  US 20190054227A1
• Time in Patent Office: 1,552 Days
• CPC Class Codes: A61M 1/0259 Apparatus for treatment of blood or blood constituents not otherwise provided for for agitating A61M1/025; for separating blood components present in distinct layers in a container A61M1/029
• CPC Class Codes: A61M 1/34 Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
• CPC Class Codes: A61M 1/3403 Regulation parameters
• CPC Class Codes: A61M 1/341 by measuring the filtrate rate or volume
• CPC Class Codes: A61M 1/3486 Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents
• CPC Class Codes: A61M 1/3609 Physical characteristics of the blood, e.g. haematocrit, urea
• CPC Class Codes: A61M 1/3633 Blood component filters, e.g. leukocyte filters
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 1/3692 Washing or rinsing blood or blood constituents
• CPC Class Codes: A61M 1/38 Removing constituents from donor blood and storing or returning remainder to body , e.g. for transfusion
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: A61P 39/02 Antidotes
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/30 Processes for preparing, regenerating, or reactivating

Abstract

An adsorbing material for multiple pathogenic factors of sepsis as well as a preparation method and an application thereof are provided. The adsorbing material is formed by coupling a carrier with good mechanical performance and blood compatibility and a ligand with the capacity to adsorb multiple pathogen-associated molecular patterns, and is capable of effectively adsorbing bacterial endotoxin, bacterial genomic DNA, peptidoglycan, lipoteichoic acid, virus RNA, and zymosan from fluids such as blood and the like, and in particular has application value in blood purification for treatment of sepsis.

Claims

1. A preparation method of a ligand of adsorbing materials for adsorbing multiple pathogenic factors of sepsis in fluids, comprising the following steps:

1) In dichloromethane, compound 1 reacts with di-tert-butyl dicarbonate to generate compound 2, reaction temperature is 20˜

30°

C., the equivalence ratio of compound 1 and di-tert-butyl dicarbonate is 1;

0.5˜

2, reaction equation is

2. The method according to claim 1, wherein the multiple pathogenic factors of sepsis include bacterial endotoxin, bacterial genomic DNA, peptidoglycan, lipoteichoic acid, virus RNA and/or zymosan.

3. The method according to claim 1, wherein the fluids include human blood or blood plasma or drug injection or liquid biological reagent.

4. An adsorbing material for multiple pathogenic factors of sepsis, wherein the material is formed by coupling the ligand prepared through the method of claim 1 and a carrier, whose molecular structure is shown as follows

5. The adsorbing material according to claim 4, wherein the carrier is amino-functionalized agarose or amino-functionalized polystyrene resin.

7. A blood purification device for treatment of sepsis comprising the adsorbing material according to claim 4.

8. A blood purification device for treatment of sepsis comprising the adsorbing material according to claim 5.

6. A preparation method of an adsorbing material for adsorbing multiple pathogenic factors of sepsis, comprising following steps:

1) In tetrahydrofuran or tetrahydrofuran aqueous solution or ethanol aqueous solution, compound 12 reacts with carrier M to generate compound 13, the mass ratio of compound 12 and carrier M is 0.01˜

1;

100, reaction equation is;